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The secondary structure indicated the presence of 12 transmembrane segments (TMSs) and characteristic motif A of MFS efflux pumps. Experimental verification of efflux activity was conducted using ethidium bromide accumulation and efflux assays and biofilm formation assays. Antimicrobial susceptibility testing and efflux pump inhibition confirmed that Nms effectively effluxed various antimicrobial agents to confer multidrug resistance. Comprehensive genomic analyses were used to assess the prevalence and possible origins of the\n              <jats:italic toggle=\"yes\">nms<\/jats:italic>\n              gene. The results revealed that the\n              <jats:italic toggle=\"yes\">nms<\/jats:italic>\n              gene was present in\n              <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n              ST398\/ST541 and\n              <jats:italic toggle=\"yes\">Staphylococcus epidermidis<\/jats:italic>\n              ST570\/ST1166 strains from global isolates. The transmission of\n              <jats:italic toggle=\"yes\">nms<\/jats:italic>\n              was associated with the prevalence of\n              <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n              ST398-t571 in swine-derived samples from China. Phylogenetic analysis revealed that\n              <jats:italic toggle=\"yes\">nms-<\/jats:italic>\n              positive strains formed a distinct clade separate from other\n              <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n              ST398 strains. Genetic analysis of the\n              <jats:italic toggle=\"yes\">nms<\/jats:italic>\n              gene revealed a significant presence of plasmid-related mobile genetic elements, with extended nucleotide sequences containing circular intermediates exhibiting high homology with those found in an\n              <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n              plasmid. These findings suggested that the\n              <jats:italic toggle=\"yes\">nms<\/jats:italic>\n              gene likely initially originated from plasmids and subsequently integrated into chromosomes. In conclusion, Nms is a novel MFS efflux pump that confers multidrug resistance to\n              <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n              and has been carried predominantly by ST398-t571 isolates in recent years. Ongoing surveillance is essential to elucidate the origin of\n              <jats:italic toggle=\"yes\">nms<\/jats:italic>\n              in\n              <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n              , particularly MRSA ST398-t571, and to understand the transmission among humans, animals, and the environment.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aac.01739-24","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T13:49:19Z","timestamp":1744033759000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Identification and characterization of a novel major facilitator superfamily (MFS) efflux pump conferring multidrug resistance in\n            <i>Staphylococcus aureus<\/i>\n            and\n            <i>Staphylococcus epidermidis<\/i>"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1939-2417","authenticated-orcid":false,"given":"Honghao","family":"Huang","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingxuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Quality Standard and Monitoring Technology for Agro-products, Guangdong Academy of Agricultural Sciences","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wan","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7679-1713","authenticated-orcid":true,"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine","place":["Hangzhou, 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