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Tons of seafood are consumed annually in China; however, whether seafood harbors tigecycline-resistant bacteria is not known. In this study, we isolated various tigecycline-resistant bacteria from retail seafood; among these,\n                      <jats:italic toggle=\"yes\">Shewanella<\/jats:italic>\n                      was the predominant tigecycline-resistant genus (33\/76, 43.4%). Genomic sequencing revealed that two\n                      <jats:italic toggle=\"yes\">Shewanella<\/jats:italic>\n                      strains carried the\n                      <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                      (X4) gene, while one\n                      <jats:italic toggle=\"yes\">Shewanella chilikensis<\/jats:italic>\n                      strain co-harbored\n                      <jats:italic toggle=\"yes\">tmexCD2-toprJ2<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>NDM-1<\/jats:sub>\n                      genes. The\n                      <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                      (X4) and\n                      <jats:italic toggle=\"yes\">tmexCD2-toprJ2<\/jats:italic>\n                      were found to be located on novel members of the SXT\/R391 family of integrated conjugative elements (ICEs). As per our knowledge, this is the first report on the emergence of SXT\/R391 ICEs carrying\n                      <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                      (X4) or\n                      <jats:italic toggle=\"yes\">tmexCD2-toprJ2<\/jats:italic>\n                      gene in\n                      <jats:italic toggle=\"yes\">Shewanella<\/jats:italic>\n                      strains. The SXT\/R391 family ICEs could mediate the spread of tigecycline resistance genes among aquatic bacteria, and contact between seafood and consumers may lead to the dissemination of tigecycline-resistant bacteria. Our study revealed that\n                      <jats:italic toggle=\"yes\">Shewanella<\/jats:italic>\n                      spp. may act as potential reservoirs of tigecycline resistance genes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01742-24","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T12:21:13Z","timestamp":1750940473000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Detection of SXT\/R391 integrative conjugative elements carrying tigecycline resistance genes in\n                    <i>Shewanella<\/i>\n                    spp. isolated from retail 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