{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:26:20Z","timestamp":1791296780936,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"FP7 Initial Training Network ParaMet","award":["290080"],"award-info":[{"award-number":["290080"]}]},{"name":"Tres Cantos Open Lab Foundation","award":["TC046"],"award-info":[{"award-number":["TC046"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The protozoan parasite\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Leishmania donovani<\/jats:named-content>\n            is the causative agent of visceral leishmaniasis, a disease potentially fatal if not treated. Current available treatments have major limitations, and new and safer drugs are urgently needed. In recent years, advances in high-throughput screening technologies have enabled the screening of millions of compounds to identify new antileishmanial agents. However, most of the compounds identified\n            <jats:italic>in vitro<\/jats:italic>\n            did not translate their activities when tested in\n            <jats:italic>in vivo<\/jats:italic>\n            models, highlighting the need to develop more predictive\n            <jats:italic>in vitro<\/jats:italic>\n            assays. In the present work, we describe the development of a robust replicative, high-content,\n            <jats:italic>in vitro<\/jats:italic>\n            intracellular\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. donovani<\/jats:named-content>\n            assay. Horse serum was included in the assay media to replace standard fetal bovine serum, to completely eliminate the extracellular parasites derived from the infection process. A novel phenotypic\n            <jats:italic>in vitro<\/jats:italic>\n            infection model has been developed, complemented with the identification of the proliferation of intracellular amastigotes measured by EdU incorporation.\n            <jats:italic>In vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo<\/jats:italic>\n            results for miltefosine, amphotericin B, and the selected compound 1 have been included to validate the assay.\n          <\/jats:p>","DOI":"10.1128\/aac.01781-15","type":"journal-article","created":{"date-parts":[[2016,3,29]],"date-time":"2016-03-29T02:23:38Z","timestamp":1459218218000},"page":"3524-3532","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["A Replicative\n            <i>In Vitro<\/i>\n            Assay for Drug Discovery against Leishmania donovani"],"prefix":"10.1128","volume":"60","author":[{"given":"Diana","family":"Tegazzini","sequence":"first","affiliation":[{"name":"Diseases of the Developing World, GlaxoSmithKline, Tres Cantos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosario","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"London School of Hygiene &amp; Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Aguilar","sequence":"additional","affiliation":[{"name":"London School of Hygiene &amp; Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imanol","family":"Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Molecular Discovery Research, GlaxoSmithKline, Tres Cantos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas L.","family":"Presa","sequence":"additional","affiliation":[{"name":"Molecular Discovery Research, GlaxoSmithKline, Tres Cantos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Yardley","sequence":"additional","affiliation":[{"name":"London School of Hygiene &amp; Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio J.","family":"Martin","sequence":"additional","affiliation":[{"name":"Molecular Discovery Research, GlaxoSmithKline, Tres Cantos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose M.","family":"Coteron","sequence":"additional","affiliation":[{"name":"Diseases of the Developing World, GlaxoSmithKline, Tres Cantos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon L.","family":"Croft","sequence":"additional","affiliation":[{"name":"London School of Hygiene &amp; Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Cantizani","sequence":"additional","affiliation":[{"name":"Diseases of the Developing World, GlaxoSmithKline, Tres Cantos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035671"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03630.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)62050-8"},{"key":"e_1_3_2_5_2","first-page":"733","article-title":"Miltefosine in the treatment of leishmaniasis: clinical evidence for informed clinical risk management","volume":"3","author":"Sundar S","year":"2007","unstructured":"Sundar S, Olliaro PL. 2007. 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