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Bacterial acquisition of MDR relies primarily on class 1 integron-involved horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs). To date, no strategies other than the use of antibiotics can efficiently cope with MDR. Here, we report that an engineered CRISPR interference (CRISPRi) system can markedly reduce MDR by blocking a class 1 integron in\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            . Using CRISPRi to block plasmid R388 class 1 integron,\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            recombinants showed halted growth upon exposure to relevant antibiotics.\n          <\/jats:p>","DOI":"10.1128\/aac.01789-19","type":"journal-article","created":{"date-parts":[[2019,12,24]],"date-time":"2019-12-24T13:53:26Z","timestamp":1577195606000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Engineering a CRISPR Interference System To Repress a Class 1 Integron in Escherichia coli"],"prefix":"10.1128","volume":"64","author":[{"given":"Qingyang","family":"Li","sequence":"first","affiliation":[{"name":"School of Food Science and Engineering, South China University of Technology, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6009-3526","authenticated-orcid":false,"given":"Haifeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering, South China University of Technology, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5261-9516","authenticated-orcid":false,"given":"Pingfang","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1145"},{"key":"e_1_3_3_3_2","volume-title":"Antibiotic resistance threats in the United States, 2013","author":"Centers for Disease Control and Prevention","year":"2013","unstructured":"Centers for Disease Control and Prevention. 2013. 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