{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:46:18Z","timestamp":1785851178071,"version":"3.56.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["7252073"],"award-info":[{"award-number":["7252073"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Clinical Key Specialty Construction Project (Intensive Care Medicine), Beijing Ditan Hospital"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Carbapenem-resistant\n                      <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                      (CRAB) poses a critical global health threat. CRAB is designated a WHO Priority 1st pathogen due to high mortality and limited therapeutic options. Meropenem, a first-line carbapenem, exhibits dose-dependent nephrotoxicity, hepatotoxicity, and neurotoxicity\u2014especially in critically ill patients\u2014necessitating strategies to reduce dosing while maintaining efficacy. Class D \u03b2-lactamase OXA23 is the predominant driver of carbapenem resistance, which is harbored by 83.4% of strains within the dominant epidemic super lineage. Critically, recent studies revealed that OXA23 can be secreted extracellularly, enhancing bacterial resistance in both CRAB and their co-colonizing gram-negative pathogens in polymicrobial environments. To counter this, we developed C32, a neutralizing monoclonal antibody targeting OXA23. C32 exhibits picomolar affinity for a conserved epitope proximal to OXA23's catalytic groove, potently neutralizing enzymatic hydrolysis and restoring carbapenem susceptibility. Functional analysis revealed that C32 reduced meropenem MICs by 33.3% (24 to 16 \u03bcg\/mL) in a CRAB co-culture model, further revealing its capacity to disrupt extracellular OXA23-mediated protection of susceptible pathogens. These findings establish C32 as a novel therapeutic strategy that overcomes OXA23-mediated resistance, offering a promising approach to revitalize CRAB infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01796-25","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T12:14:48Z","timestamp":1782389688000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["A monoclonal antibody targeting OXA23 restores carbapenem susceptibility in global epidemic carbapenem-resistant\n                    <i>Acinetobacter baumannii<\/i>"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2525-6822","authenticated-orcid":true,"given":"Mingxi","family":"Hua","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013xs5b60","id-type":"ROR","asserted-by":"publisher"}],"name":"Biomedical Innovation Center, 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