{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T22:03:23Z","timestamp":1794002603982,"version":"4.8.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Chitosan and nanoparticle silver are both materials with demonstrated antimicrobial properties and have been proposed singly or in combination as constituents of antimicrobial burn dressings. Here, we show that they combine synergistically to inhibit the<jats:italic>in vitro<\/jats:italic>growth of Gram-positive methicillin-resistant<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>(MRSA) and Gram-negative bacteria (<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas aeruginosa<\/jats:named-content>,<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Proteus mirabilis<\/jats:named-content>, and<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter baumannii<\/jats:named-content>), as judged by bioluminescence monitoring and isobolographic analysis, and also produce synergistic killing after 30 min of incubation, as measured by a CFU assay. The hypothesized explanation involves chitosan-mediated permeabilization of bacterial cells, allowing better penetration of silver ions into the cell. A dressing composed of freeze-dried chitosan acetate incorporating nanoparticle silver was compared with a dressing of chitosan acetate alone in an<jats:italic>in vivo<\/jats:italic>burn model infected with bioluminescent<jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>. The survival rates of mice treated with silver-chitosan or regular chitosan or left untreated were 64.3% (<jats:italic>P<\/jats:italic>= 0.0082 versus regular chitosan and<jats:italic>P<\/jats:italic>= 0.0003 versus the control), 21.4%, and 0%, respectively. Most of the fatalities occurred between 2 and 5 days postinfection. Silver-chitosan dressings effectively controlled the development of systemic sepsis, as shown by blood culture. These data suggest that a dressing combining chitosan acetate with silver leads to improved antimicrobial efficacy against fatal burn infections.<\/jats:p>","DOI":"10.1128\/aac.01803-10","type":"journal-article","created":{"date-parts":[[2011,4,19]],"date-time":"2011-04-19T04:25:10Z","timestamp":1303187110000},"page":"3432-3438","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":151,"title":["Synergistic Combination of Chitosan Acetate with Nanoparticle Silver as a Topical Antimicrobial: Efficacy against Bacterial Burn Infections"],"prefix":"10.1128","volume":"55","author":[{"given":"Liyi","family":"Huang","sequence":"first","affiliation":[{"name":"Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts"},{"name":"Department of Dermatology, Harvard Medical School, Boston, Massachusetts"},{"name":"Department of Infectious Diseases, First Affiliated College and Hospital, Guangxi Medical University, Nanning, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhong","family":"Dai","sequence":"additional","affiliation":[{"name":"Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts"},{"name":"Department of Dermatology, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Xuan","sequence":"additional","affiliation":[{"name":"Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts"},{"name":"School of Engineering, Tufts University, Medford, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George P.","family":"Tegos","sequence":"additional","affiliation":[{"name":"Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts"},{"name":"Department of Dermatology, Harvard Medical School, Boston, Massachusetts"},{"name":"Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Hamblin","sequence":"additional","affiliation":[{"name":"Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts"},{"name":"Department of Dermatology, Harvard Medical School, Boston, Massachusetts"},{"name":"Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0031-6997(25)00026-2","article-title":"What is synergy?","volume":"41","author":"Berenbaum M. 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