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Previous studies have shown that the expression of\n            <jats:italic>erm<\/jats:italic>\n            is activated when a macrolide-bound ribosome stalls the translation of the leader peptide preceding the cotranscribed\n            <jats:italic>erm<\/jats:italic>\n            . Ribosome stalling is thought to destabilize the inhibitory stem-loop mRNA structure and exposes the\n            <jats:italic>erm<\/jats:italic>\n            Shine-Dalgarno (SD) sequence for translational initiation. Paradoxically, mutations that abolish ribosome stalling are routinely found in hyper-resistant clinical isolates; however, the significance of the stalling-dead leader sequence is largely unknown. Here, we show that nonsense mutations in the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>\n            ErmB leader peptide (ErmBL) lead to high basal and induced expression of downstream ErmB in the absence or presence of macrolide concomitantly with elevated ribosome methylation and resistance. The overexpression of ErmB is associated with the reduced turnover of the\n            <jats:italic>ermBL-ermB<\/jats:italic>\n            transcript, and the macrolide appears to mitigate mRNA cleavage at a site immediately downstream of the\n            <jats:italic>ermBL<\/jats:italic>\n            SD sequence. The stabilizing effect of antibiotics on mRNA is not limited to\n            <jats:italic>ermBL-ermB<\/jats:italic>\n            ; cationic antibiotics representing a ribosome-stalling inducer and a noninducer increase the half-life of specific transcripts. These data unveil a new layer of\n            <jats:italic>ermB<\/jats:italic>\n            regulation and imply that ErmBL translation or ribosome stalling serves as a \u201ctuner\u201d to suppress aberrant production of ErmB because methylated ribosome may impose a fitness cost on the bacterium as a result of misregulated translation.\n          <\/jats:p>","DOI":"10.1128\/aac.01806-16","type":"journal-article","created":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T01:59:47Z","timestamp":1474336787000},"page":"7178-7188","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["The Expression of Antibiotic Resistance Methyltransferase Correlates with mRNA Stability Independently of Ribosome Stalling"],"prefix":"10.1128","volume":"60","author":[{"given":"Ekaterina","family":"Dzyubak","sequence":"first","affiliation":[{"name":"Edward A. 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