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This microbe readily exhibits intrinsic and acquired resistance to varied antimicrobial drugs. Resistance to penicillin-like compounds is commonplace and provided by the chromosomal AmpC \u03b2-lactamase. A second, chromosomally encoded \u03b2-lactamase, PoxB, has previously been reported in\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. aeruginosa<\/jats:named-content>\n                    . In the present work, the contribution of this class D enzyme was investigated using a series of clean in-frame\n                    <jats:italic>ampC<\/jats:italic>\n                    ,\n                    <jats:italic>poxB<\/jats:italic>\n                    , and\n                    <jats:italic>oprD<\/jats:italic>\n                    deletions, as well as complementation by expression under the control of an inducible promoter. While\n                    <jats:italic>poxB<\/jats:italic>\n                    deletions failed to alter \u03b2-lactam sensitivities, expression of\n                    <jats:italic>poxB<\/jats:italic>\n                    in\n                    <jats:italic>ampC<\/jats:italic>\n                    -deficient backgrounds decreased susceptibility to both meropenem and doripenem but had no effect on imipenem, penicillin, and cephalosporin MICs. However, when expressed in an\n                    <jats:italic>ampCpoxB<\/jats:italic>\n                    -deficient background, that additionally lacked the outer membrane porin-encoding gene\n                    <jats:italic>oprD<\/jats:italic>\n                    , PoxB significantly increased the imipenem as well as the meropenem and doripenem MICs. Like other class D carbapenem-hydrolyzing \u03b2-lactamases, PoxB was only poorly inhibited by class A enzyme inhibitors, but a novel non-\u03b2-lactam compound, avibactam, was a slightly better inhibitor of PoxB activity.\n                    <jats:italic>In vitro<\/jats:italic>\n                    susceptibility testing with a clinical concentration of avibactam, however, failed to reduce PoxB activity against the carbapenems. In addition,\n                    <jats:italic>poxB<\/jats:italic>\n                    was found to be cotranscribed with an upstream open reading frame,\n                    <jats:italic>poxA<\/jats:italic>\n                    , which itself was shown to encode a 32-kDa protein of yet unknown function.\n                  <\/jats:p>","DOI":"10.1128\/aac.01807-15","type":"journal-article","created":{"date-parts":[[2015,11,30]],"date-time":"2015-11-30T22:39:48Z","timestamp":1448923188000},"page":"936-945","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Characterization of a Carbapenem-Hydrolyzing Enzyme, PoxB, in Pseudomonas aeruginosa PAO1"],"prefix":"10.1128","volume":"60","author":[{"given":"Diansy","family":"Zincke","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, College of Arts and Sciences, Florida International University, Miami, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak","family":"Balasubramanian","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Arts and Sciences, Florida International University, Miami, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn L.","family":"Silver","sequence":"additional","affiliation":[{"name":"LL Silver Consulting, Springfield, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalai","family":"Mathee","sequence":"additional","affiliation":[{"name":"Department of Human and Molecular Genetics, Herbert Wertheim College of Medicine, Florida International University, Miami, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200767030-00003"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/5.2.279"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/07357909209032787"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.160.4.501"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/14.2.403"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199814000-00008"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1306801"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/668770"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)92422-P"},{"key":"e_1_3_3_11_2","volume-title":"Cystic Fibrosis Foundation patient registry 2012","author":"Cystic Fibrosis Foundation","year":"2012","unstructured":"Cystic Fibrosis Foundation. 2012. 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