{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:46:55Z","timestamp":1785851215889,"version":"3.56.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Fund for Scientific Research Flanders","award":["1181818N"],"award-info":[{"award-number":["1181818N"]}]},{"name":"Micreos Human Health b.v."},{"name":"FWO biofilm research community on the ecology of bacterial and fungal biofilms in humans","award":["W000921N"],"award-info":[{"award-number":["W000921N"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The oral cavity is colonized by many species of bacteria and fungi without causing serious infections of hosts. However, upon loss of homeostasis, some of these colonizers have the potential to invade the host and cause life-threatening infections. The fungus\n                      <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                      and the gram-positive bacterium\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      represent well-studied examples. In immunocompromised mice, oral co-colonization with these two species can result in dissemination of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      through the body, causing\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      bacteremia and subsequent sepsis. In the proof-of-principle experiments described here, we show effective prevention of dissemination of methicillin-resistant\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      (MRSA) using an innovative endolysin-derived chimeric protein, XZ.700. Using the established murine model for oral co-colonization, we evaluated the effect of treatment with XZ.700 in drinking water (\n                      <jats:italic toggle=\"yes\">ad libitum<\/jats:italic>\n                      ) and applied as a gel formulation twice a day. XZ.700 did not show any adverse reaction in the mice treated and did not show a reduction of\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      colonization. XZ.700 in drinking water completely prevented detectable dissemination of MRSA in co-infected mice and significantly reduced weight loss. The gel formulation showed a trend in decreased MRSA colonization of the tongue and dissemination to the kidneys, although this was not significantly different from the placebo control. Taken together, the results presented here indicate that selective removal of MRSA from the oral cavity of mice with XZ.700 during co-infection with\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      is effective in preventing dissemination. Further studies need to show clinical applicability of XZ.700 and related compounds in prevention of sepsis in at-risk patients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01812-25","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T15:03:01Z","timestamp":1781535781000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Phage-derived endolysin prevents dissemination of methicillin resistant\n                    <i>Staphylococcus aureus<\/i>\n                    during oral co-infection with\n                    <i>Candida albicans<\/i>"],"prefix":"10.1128","volume":"70","author":[{"given":"Katrien Van","family":"Dyck","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05f950310","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory of Molecular Cell Biology, Department of Biology, Institute of Botany and 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