{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:51:09Z","timestamp":1789696269746,"version":"4.0.1"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003593","name":"MCTI | Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["402787\/2013-7"],"award-info":[{"award-number":["402787\/2013-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Conventional chemotherapy of cutaneous leishmaniasis (CL) is based on multiple parenteral or intralesional injections with systemically toxic drugs. Aiming at a single-dose localized therapy, biodegradable poly(lactic-co-glycolic acid) (PLGA) microparticles loaded with 7.8% of an antileishmanial nitrochalcone named CH8 (CH8\/PLGA) were constructed to promote sustained subcutaneous release.\n            <jats:italic>In vitro<\/jats:italic>\n            , murine macrophages avidly phagocytosed CH8\/PLGA smaller than 6 \u03bcm without triggering oxidative mechanisms. Upon 48 h of incubation, both CH8 and CH8\/PLGA were 40 times more toxic to intracellular\n            <jats:named-content content-type=\"genus-species\">Leishmania amazonensis<\/jats:named-content>\n            than to macrophages.\n            <jats:italic>In vivo<\/jats:italic>\n            , BALB\/c were given one or three subcutaneous injections in the infected ear with 1.2 mg\/kg of CH8 in free or CH8\/PLGA forms, whereas controls received three CH8-equivalent doses of naked PLGA microparticles or meglumine antimoniate (Glucantime; Sanofi-Aventis). Although a single injection with CH8\/PLGA reduced the parasite loads by 91%, triple injections with free CH8 or CH8\/PLGA caused 80 and 97% reductions, respectively, in relation to saline controls. Meglumine antimoniate treatment was the least effective (only 36% reduction) and the most toxic, as indicated by elevated alanine aminotransferase serum levels. Together, these findings show that CH8\/PLGA microparticles can be effectively and safely used for single-dose treatment of CL.\n          <\/jats:p>","DOI":"10.1128\/aac.01822-17","type":"journal-article","created":{"date-parts":[[2018,2,22]],"date-time":"2018-02-22T11:28:34Z","timestamp":1519298914000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Depot Subcutaneous Injection with Chalcone CH8-Loaded Poly(Lactic-Co-Glycolic Acid) Microspheres as a Single-Dose Treatment of Cutaneous Leishmaniasis"],"prefix":"10.1128","volume":"62","author":[{"given":"Ariane de Jesus","family":"Sousa-Batista","sequence":"first","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wallace","family":"Pacienza-Lima","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Arruda-Costa","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Alves Bandeira","family":"Falc\u00e3o","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ines","family":"R\u00e9","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Toulouse, Mines Albi, CNRS, Centre Rapsodee, Campus Jarlard, F-81013 Albi Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartira","family":"Rossi-Bergmann","sequence":"additional","affiliation":[{"name":"Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035671"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1752-1947-7-31"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0149697"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2015.03.018"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2222-1808(15)60975-6"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2015.973850"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000381"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00163"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0625.2000.009003165.x"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.09.032"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.11-0612"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5154.143571"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2012.05460.x"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/dth.12377"},{"key":"e_1_3_1_16_2","volume-title":"Manual de vigil\u00e2ncia da leishmaniose tegumentar recurso eletr\u00f4nico","author":"Minist\u00e9rio da Sa\u00fade, Secretaria de Vigil\u00e2ncia em Sa\u00fade","year":"2017","unstructured":"Minist\u00e9rio da Sa\u00fade, Secretaria de Vigil\u00e2ncia em Sa\u00fade. 2017. 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