{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:21:48Z","timestamp":1787062908650,"version":"build-2736575974"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Infectious Disease Medical Center startup fund","award":["B2022011-1"],"award-info":[{"award-number":["B2022011-1"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2026,5,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Invasive pulmonary aspergillosis (IPA) caused by\n                      <jats:italic toggle=\"yes\">Aspergillus fumigatus<\/jats:italic>\n                      remains a clinical challenge due to limited therapies and rising antifungal resistance. Here, we evaluated nitroxoline (NTX), an FDA-approved 8-hydroxyquinoline derivative, for its antifungal potential and mechanism of action. NTX exhibited potent\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      activity against 64 clinical isolates and the AF293 reference strain, delaying conidial maturation and significantly improving survival, reducing pulmonary fungal burden, and alleviating lung inflammation and hyphal invasion in an IPA mouse model. Transcriptomic analysis revealed marked downregulation of the high-affinity Cu transporter\n                      <jats:italic toggle=\"yes\">ctrC<\/jats:italic>\n                      and superoxide dismutase\n                      <jats:italic toggle=\"yes\">sodB<\/jats:italic>\n                      , disrupting the copper homeostasis-oxidative stress axis. Copper supplementation restored hyphal growth and intracellular ROS levels, supporting a mechanism of copper deprivation-induced oxidative stress. These findings demonstrate that NTX exerts strong anti-\n                      <jats:italic toggle=\"yes\">A<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">fumigatus<\/jats:italic>\n                      effects\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      by perturbing copper homeostasis and inducing oxidative stress, highlighting its unique antifungal potential and providing a conceptual basis for metal-targeted therapeutic strategies against IPA and drug-resistant fungal infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01831-25","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T14:41:56Z","timestamp":1774968116000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Nitroxoline exerts potent anti-\n                    <i>Aspergillus fumigatus<\/i>\n                    activity by disrupting copper homeostasis and inducing oxidative stress"],"prefix":"10.1128","volume":"70","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2568-7721","authenticated-orcid":true,"given":"Yiru","family":"Zhang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0232r4451","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, China-Singapore Belt and Road Joint Laboratory on Infection Research and Drug Development, National Medical 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