{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:45:55Z","timestamp":1792025155148,"version":"4.4.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,6,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      We analyzed antimicrobial susceptibilities of 415\n                      <jats:italic toggle=\"yes\">Ureaplasma<\/jats:italic>\n                      isolates from various sample types derived from different regions of the United States and Canada from 2012 to 2023 and investigated the genetic mechanisms of antimicrobial resistance. Minimum inhibitory concentration (MIC) ranges for erythromycin, tetracycline, and levofloxacin were 0.063\u2013256, 0.016\u201364, and 0.063\u201332 \u00b5g\/mL, respectively. MIC\n                      <jats:sub>50<\/jats:sub>\n                      values for erythromycin, tetracycline, and levofloxacin were 2, 0.25, and 1 \u00b5g\/mL, and MIC\n                      <jats:sub>90<\/jats:sub>\n                      values were 4, 1, and 2 \u00b5g\/mL, respectively. According to Clinical and Laboratory Standards Institute breakpoints, there were 61 (14.7%) isolates resistant to one or more drugs, and resistance rates for erythromycin, tetracycline, and levofloxacin were 2.4% (10\/415), 6.5% (27\/413), and 6.7% (28\/415), respectively. Four isolates (1.0%) were resistant to two drugs. Mutations in domain V of 23S rRNA, mainly A2058G (\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      numbering), and\/or in the\n                      <jats:italic toggle=\"yes\">rpl<\/jats:italic>\n                      D gene encoding ribosomal protein L4 were identified in most erythromycin-resistant isolates.\n                      <jats:italic toggle=\"yes\">Tet<\/jats:italic>\n                      (M) was detected in all isolates with tetracycline MIC \u22654 \u00b5g\/mL but absent in 64.7% (11\/17) of isolates with MIC of 2 \u00b5g\/mL. For fluoroquinolone-resistant isolates, C248T (S83L) and G259A (E87K) mutations in\n                      <jats:italic toggle=\"yes\">parC<\/jats:italic>\n                      were identified in most cases (23\/26). In summary, erythromycin, tetracycline, and levofloxacin are still effective\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      against most\n                      <jats:italic toggle=\"yes\">Ureaplasma<\/jats:italic>\n                      isolates in North America.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01868-24","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T11:09:51Z","timestamp":1746443391000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Antimicrobial susceptibility and genetic mechanisms of resistance of\n                    <i>Ureaplasma<\/i>\n                    isolates in North America between 2012 and 2023"],"prefix":"10.1128","volume":"69","author":[{"given":"Joshua T.","family":"Waites","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008s83205","id-type":"ROR","asserted-by":"publisher"}],"name":"Departments of Pathology, The University of Alabama at Birmingham","place":["Birmingham, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna M.","family":"Crabb","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008s83205","id-type":"ROR","asserted-by":"publisher"}],"name":"Departments of Pathology, The University of Alabama at Birmingham","place":["Birmingham, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy E.","family":"Ratliff","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008s83205","id-type":"ROR","asserted-by":"publisher"}],"name":"Departments of Pathology, The University of Alabama at Birmingham","place":["Birmingham, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8775-765X","authenticated-orcid":true,"given":"Ken B.","family":"Waites","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008s83205","id-type":"ROR","asserted-by":"publisher"}],"name":"Departments of Pathology, The University of Alabama at Birmingham","place":["Birmingham, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0188-4023","authenticated-orcid":true,"given":"Li","family":"Xiao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008s83205","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, The University of Alabama at Birmingham","place":["Birmingham, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","volume-title":"Manual of clinical microbiology","author":"Waites KB","year":"2023","unstructured":"Waites KB, Bebear CM. 2023. Mycoplasma and Ureaplasma. In Carroll KC, Pfaller M, Karlowsky JA, Landry ML, McAdam AJ, Patel R, BS P (ed), Manual of clinical microbiology, 13th ed. ASM Press, Washington, D.C.","edition":"13"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa8419"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2007.07.033"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-5347(17)45953-2"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-024-02435-2"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(88)92536-6"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2009.040394"},{"key":"e_1_3_4_9_2","first-page":"289","volume-title":"Mollicutes molecular biology and pathogenesis","author":"Waites KB","year":"2014","unstructured":"Waites KB, Lysynyansky I, Bebear CM. 2014. Antibiotic susceptibility testing and resistance in mycoplasmas of humans and animals, p 289\u2013322. In Browning G, Citti C (ed), Mollicutes molecular biology and pathogenesis. Caister Academic Press, Norfolk, UK."},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06342-11"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.12.012"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky238"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab320"},{"key":"e_1_3_4_14_2","first-page":"225","article-title":"Antibiotic susceptibilities and genetic variations in macrolide resistance genes of Ureaplasma spp. isolated in China","volume":"42","author":"Song T","year":"2019","unstructured":"Song T, Huang J, Liu Z, Zhang Y, Kong Y, Ruan Z. 2019. Antibiotic susceptibilities and genetic variations in macrolide resistance genes of Ureaplasma spp. isolated in China. 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