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In a bile sample, we identified three extended-spectrum-\u03b2-lactamase (ESBL)-producing bacteria (\n Escherichia coli<\/jats:named-content>\n ,\n Klebsiella pneumoniae<\/jats:named-content>\n , and\n Shewanella<\/jats:named-content>\n sp. strain JAB-1) isolated from a child suffering from cholangitis. Our objectives were to characterize the genome and the resistome of the first ESBL-producing isolate of the genus\n Shewanella<\/jats:named-content>\n and determine whether plasmidic exchange occurred between the three bacterial species. Bacterial isolates were characterized using matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry (MALDI-TOF MS), standard biochemical tools, and antimicrobial susceptibility testing.\n Shewanella<\/jats:named-content>\n sp. JAB-1 and ESBL gene-encoding plasmids were characterized using PacBio and Illumina whole-genome sequencing, respectively. The\n Shewanella<\/jats:named-content>\n sp. JAB-1 chromosome-encoded OXA-48 variant was cloned and functionally characterized. Whole-genome sequencing (WGS) of the\n Shewanella<\/jats:named-content>\n sp. clinical isolate JAB-1 revealed the presence of a 193-kb plasmid belonging to the IncA\/C incompatibility group and harboring two ESBL genes,\n bla<\/jats:italic>\n CTX-M-15<\/jats:sub>\n and\n bla<\/jats:italic>\n SHV-2a<\/jats:sub>\n .\n bla<\/jats:italic>\n CTX-M-15<\/jats:sub>\n gene-carrying plasmids belonging to the IncY and IncR incompatibility groups were also found in the\n E. coli<\/jats:named-content>\n and\n K. pneumoniae<\/jats:named-content>\n isolates from the same patient, respectively. A comparison of the\n bla<\/jats:italic>\n CTX-M-15<\/jats:sub>\n genetic environment indicated the independent origin of these plasmids and dismissed\n in vivo<\/jats:italic>\n transfers. Furthermore, characterization of the resistome of\n Shewanella<\/jats:named-content>\n sp. JAB-1 revealed the presence of a chromosome-carried\n bla<\/jats:italic>\n OXA-535<\/jats:sub>\n gene, likely the progenitor of the plasmid-carried\n bla<\/jats:italic>\n OXA-436<\/jats:sub>\n gene, a novel\n bla<\/jats:italic>\n OXA-48<\/jats:sub>\n -like gene. The expression of\n bla<\/jats:italic>\n OXA-535<\/jats:sub>\n in\n E. coli<\/jats:named-content>\n showed the carbapenem-hydrolyzing activity of OXA-535. The production of OXA-535 in\n Shewanella<\/jats:named-content>\n sp. JAB-1 could be evidenced using molecular and immunoenzymatic tests, but not with biochemical tests that monitor carbapenem hydrolysis. In this study, we have identified a CTX-M-15-producing\n Shewanella<\/jats:named-content>\n species that was responsible for a hepatobiliary infection and that is likely the progenitor of OXA-436, a novel plasmid-encoded OXA-48-like class D carbapenemase.\n <\/jats:p>","DOI":"10.1128\/aac.01879-17","type":"journal-article","created":{"date-parts":[[2017,10,17]],"date-time":"2017-10-17T00:55:24Z","timestamp":1508201724000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["CTX-M-15-Producing Shewanella Species Clinical Isolate Expressing OXA-535, a Chromosome-Encoded OXA-48 Variant, Putative Progenitor of the Plasmid-Encoded OXA-436"],"prefix":"10.1128","volume":"62","author":[{"given":"Agn\u00e8s B.","family":"Jousset","sequence":"first","affiliation":[{"name":"Department of Bacteriology-Parasitology-Hygiene, Bic\u00eatre Hospital, Assistance Publique-H\u00f4pitaux de Paris, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance, Le Kremlin-Bic\u00eatre, France"},{"name":"EA7361 Structure, dynamic, function and expression of broad spectrum \u03b2-lactamases, Paris-Sud University, Faculty of Medicine, Le Kremlin-Bic\u00eatre, France"},{"name":"Joint Research Unit Evolution and Ecology of Resistance to Antibiotics (EERA), Institut Pasteur-APHP-University Paris Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Dabos","sequence":"additional","affiliation":[{"name":"EA7361 Structure, dynamic, function and expression of broad spectrum \u03b2-lactamases, Paris-Sud University, Faculty of Medicine, Le Kremlin-Bic\u00eatre, France"},{"name":"Joint Research Unit Evolution and Ecology of Resistance to Antibiotics (EERA), Institut Pasteur-APHP-University Paris Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9my A.","family":"Bonnin","sequence":"additional","affiliation":[{"name":"Associated French National Reference Center for Antibiotic Resistance, Le Kremlin-Bic\u00eatre, France"},{"name":"EA7361 Structure, dynamic, function and expression of broad spectrum \u03b2-lactamases, Paris-Sud University, Faculty of Medicine, Le Kremlin-Bic\u00eatre, France"},{"name":"Joint Research Unit Evolution and Ecology of Resistance to Antibiotics (EERA), Institut Pasteur-APHP-University Paris Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Girlich","sequence":"additional","affiliation":[{"name":"EA7361 Structure, dynamic, function and expression of broad spectrum \u03b2-lactamases, Paris-Sud University, Faculty of Medicine, Le Kremlin-Bic\u00eatre, France"},{"name":"Joint Research Unit Evolution and Ecology of Resistance to Antibiotics (EERA), Institut Pasteur-APHP-University Paris Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana\u00efs","family":"Potron","sequence":"additional","affiliation":[{"name":"Department of Bacteriology-Parasitology-Hygiene, CHU Besan\u00e7on, Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Cabanel","sequence":"additional","affiliation":[{"name":"Joint Research Unit Evolution and Ecology of Resistance to Antibiotics (EERA), Institut Pasteur-APHP-University Paris Sud, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6596-7384","authenticated-orcid":false,"given":"Laurent","family":"Dortet","sequence":"additional","affiliation":[{"name":"Department of Bacteriology-Parasitology-Hygiene, Bic\u00eatre Hospital, Assistance Publique-H\u00f4pitaux de Paris, Le Kremlin-Bic\u00eatre, France"},{"name":"Associated French National Reference Center for Antibiotic Resistance, Le Kremlin-Bic\u00eatre, 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