{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T17:02:06Z","timestamp":1789405326934,"version":"build-2803163510"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R21-AI-146503"],"award-info":[{"award-number":["1R21-AI-146503"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Science and Technology in Dengue and Host-Microorganism Interaction","award":["CNPQ: 465425 \/2014-3"],"award-info":[{"award-number":["CNPQ: 465425 \/2014-3"]}]},{"name":"Fundacao de Amparo a Pesquisa do Estado de Minas Gerais","award":["BPD-01010-22,6205283B-BB28-4F9C-AA65-808FE4450542"],"award-info":[{"award-number":["BPD-01010-22,6205283B-BB28-4F9C-AA65-808FE4450542"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P51 OD011092, R42 AI155275"],"award-info":[{"award-number":["P51 OD011092, R42 AI155275"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,5,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Yellow fever virus (YFV) is a deadly zoonotic flavivirus endemic in tropical\/sub-tropical Africa and South America transmitted by mosquito vector (\n                      <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                      ;\n                      <jats:italic toggle=\"yes\">Haemagogus leucocelaenus<\/jats:italic>\n                      ) to humans and non-human primates. There are no approved antiviral agents for YFV. We previously identified 7-deaza-7-fluoro-2\u2032-C-methyladenosine (DFA) with anti-YFV activity. Interestingly, DFA exhibits pan-activity in vitro against flaviviruses, such as dengue, Japanese encephalitis, Zika, and hepatitis C. This study aimed to expand DFA\u2019s anti-flavivirus profile. DFA exhibited potent sub-micromolar anti-YFV activity\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      against both the vaccine strain (YFV-17D) and a viscerotropic clinical YFV isolate (DakH1279) concomitantly with low cellular cytotoxicity and no notable mitochondrial toxicity.\n                      <jats:italic toggle=\"yes\">In vivo<\/jats:italic>\n                      , efficacy was assessed against both YFV-17DD and a human clinical isolate in A129 and AG129 mouse flavivirus infection models, respectively. DFA significantly reduced virus replication in the livers of YFV-infected mice and the hallmarks of YFV-induced liver damage, including alanine transaminase levels and indocyanine green clearance. Collectively, this work identifies DFA as a potent YFV inhibitor and lays the groundwork for further therapeutic development as a YFV and, potentially, pan-flavivirus therapeutic.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01889-24","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T09:02:51Z","timestamp":1744621371000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Combating yellow fever virus with 7-deaza-7-fluoro-2\u2032-C-methyladenosine"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0056-176X","authenticated-orcid":true,"given":"Julia C.","family":"LeCher","sequence":"first","affiliation":[{"name":"Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine and Children\u2019s Healthcare of Atlanta","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivian Vasconcelos","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Morphology, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais","place":["Belo Horizonte, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren N.","family":"Rust","sequence":"additional","affiliation":[{"name":"Division of Pathobiology & Immunology, Oregon National Primate Research Center, Oregon Health & Science University","place":["Portland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leda C.","family":"Bassit","sequence":"additional","affiliation":[{"name":"Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine and Children\u2019s Healthcare of Atlanta","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1601-256X","authenticated-orcid":true,"given":"Dharmeshkumar","family":"Patel","sequence":"additional","affiliation":[{"name":"Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine and Children\u2019s Healthcare of Atlanta","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar","family":"Rezaei","sequence":"additional","affiliation":[{"name":"Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine and Children\u2019s Healthcare of Atlanta","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"Moua","sequence":"additional","affiliation":[{"name":"Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine and Children\u2019s Healthcare of Atlanta","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Rocha da Silva","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais","place":["Belo Horizonte, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus Rodrigues","family":"Goncalves","sequence":"additional","affiliation":[{"name":"Department of Morphology, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais","place":["Belo Horizonte, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso Martins","family":"Queroz-Junior","sequence":"additional","affiliation":[{"name":"Department of Morphology, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais","place":["Belo Horizonte, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Martins","family":"Marim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais","place":["Belo Horizonte, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longhu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine and Children\u2019s Healthcare of Atlanta","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujin","family":"Lee","sequence":"additional","affiliation":[{"name":"Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine and Children\u2019s Healthcare of Atlanta","place":["Atlanta, 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