{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:32:57Z","timestamp":1787790777406,"version":"build-2784847793"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The generation of a new antifungal against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Candida albicans<\/jats:named-content>\n            biofilms has become a major priority, since biofilm formation by this opportunistic pathogenic fungus is usually associated with an increased resistance to azole antifungal drugs and treatment failures. Miltefosine is an alkyl phospholipid with promising antifungal activity. Here, we report that, when tested under planktonic conditions, miltefosine displays potent\n            <jats:italic>in vitro<\/jats:italic>\n            activity against multiple fluconazole-susceptible and -resistant\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. albicans<\/jats:named-content>\n            clinical isolates, including isolates overexpressing efflux pumps and\/or with well-characterized Erg11 mutations. Moreover, miltefosine inhibits\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. albican<\/jats:named-content>\n            s biofilm formation and displays activity against preformed biofilms. Serial passage experiments confirmed that miltefosine has a reduced potential to elicit resistance, and screening of a library of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. albicans<\/jats:named-content>\n            transcription factor mutants provided additional insight into the activity of miltefosine against\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. albicans<\/jats:named-content>\n            growing under planktonic and biofilm conditions. Finally, we demonstrate the\n            <jats:italic>in vivo<\/jats:italic>\n            efficacy of topical treatment with miltefosine in the murine model of oropharyngeal candidiasis. Overall, our results confirm the potential of miltefosine as a promising antifungal drug candidate, in particular for the treatment of azole-resistant and biofilm-associated superficial candidiasis.\n          <\/jats:p>","DOI":"10.1128\/aac.01890-15","type":"journal-article","created":{"date-parts":[[2015,9,29]],"date-time":"2015-09-29T02:38:25Z","timestamp":1443494305000},"page":"7611-7620","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["<i>In Vitro<\/i>\n            Activity of Miltefosine against Candida albicans under Planktonic and Biofilm Growth Conditions and\n            <i>In Vivo<\/i>\n            Efficacy in a Murine Model of Oral Candidiasis"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5600-4367","authenticated-orcid":false,"given":"Taissa Vieira Machado","family":"Vila","sequence":"first","affiliation":[{"name":"Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok K.","family":"Chaturvedi","sequence":"additional","affiliation":[{"name":"Department of Biology, The University of Texas at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Rozental","sequence":"additional","affiliation":[{"name":"Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose L.","family":"Lopez-Ribot","sequence":"additional","affiliation":[{"name":"Department of Biology, The University of Texas at San Antonio, San Antonio, Texas, USA"},{"name":"South Texas Center for Emerging Infectious Diseases, The University of Texas at San Antonio, San Antonio, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.2.255-267.2004"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01333-14"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000828"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050001"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/421946"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059373"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"2429","DOI":"10.1001\/archinte.1995.00430220087009","article-title":"Therapeutic approaches in patients with candidemia: evaluation in a multicenter, prospective, observational study","volume":"155","author":"Nguyen MH","year":"1995","unstructured":"Nguyen MH, Peacock JEJ, Tanner DC, Morris AJ, Nguyen ML, Snydman DR, Wagener MM, Yu VL. 1995. 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