{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:06:29Z","timestamp":1792181189885,"version":"4.4.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"University Grants Commission, New Delhi, India","award":["F1-17.1\/2013-2014\/MANF-2013-14-SIK-CHA-22060\/(SA-III\/Website)"],"award-info":[{"award-number":["F1-17.1\/2013-2014\/MANF-2013-14-SIK-CHA-22060\/(SA-III\/Website)"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa<\/jats:named-content>is a multifaceted pathogen causing a variety of biofilm-mediated infections, including catheter-associated urinary tract infections (CAUTIs). The high prevalence of CAUTIs in hospitals, their clinical manifestations, such as urethritis, cystitis, pyelonephritis, meningitis, urosepsis, and death, and the associated economic challenges underscore the need for management of these infections. Biomaterial modification of urinary catheters with two drugs seems an interesting approach to combat CAUTIs by inhibiting biofilm. Previously, we demonstrated the<jats:italic>in vitro<\/jats:italic>efficacy of urinary catheters impregnated with azithromycin (AZM) and ciprofloxacin (CIP) against<jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>. Here, we report how these coated catheters impact the course of CAUTI induced by<jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>in a murine model. CAUTI was established in female LACA mice with uncoated or AZM-CIP-coated silicone implants in the bladder, followed by transurethral inoculation of 10<jats:sup>8<\/jats:sup>CFU\/ml of biofilm cells of<jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>PAO1. AZM-CIP-coated implants (i) prevented biofilm formation on the implant's surface (<jats:italic>P<\/jats:italic>\u2264 0.01), (ii) restricted bacterial colonization in the bladder and kidney (<jats:italic>P<\/jats:italic>&lt; 0.0001), (iii) averted bacteriuria (<jats:italic>P<\/jats:italic>&lt; 0.0001), and (iv) exhibited no major histopathological changes for 28 days in comparison to uncoated implants, which showed persistent CAUTI. Antibiotic implants also overcame implant-mediated inflammation, as characterized by trivial levels of inflammatory markers such as malondialdehyde (<jats:italic>P<\/jats:italic>&lt; 0.001), myeloperoxidase (<jats:italic>P<\/jats:italic>&lt; 0.05), reactive oxygen species (<jats:italic>P<\/jats:italic>\u2264 0.001), and reactive nitrogen intermediates (<jats:italic>P<\/jats:italic>&lt; 0.01) in comparison to those in uncoated implants. Further, AZM-CIP-coated implants showed immunomodulation by manipulating the release of inflammatory cytokines interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-\u03b1), and IL-10 to the benefit of the host. Overall, the study demonstrates long-term<jats:italic>in vivo<\/jats:italic>effectiveness of AZM-CIP-impregnated catheters, which may possibly be a key to success in preventing CAUTIs.<\/jats:p>","DOI":"10.1128\/aac.01906-16","type":"journal-article","created":{"date-parts":[[2016,12,29]],"date-time":"2016-12-29T02:30:21Z","timestamp":1482978621000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Azithromycin-Ciprofloxacin-Impregnated Urinary Catheters Avert Bacterial Colonization, Biofilm Formation, and Inflammation in a Murine Model of Foreign-Body-Associated Urinary Tract Infections Caused by Pseudomonas aeruginosa"],"prefix":"10.1128","volume":"61","author":[{"given":"Hina","family":"Saini","sequence":"first","affiliation":[{"name":"Department of Microbiology, Panjab University, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anitha","family":"Vadekeetil","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Panjab University, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2600-1450","authenticated-orcid":false,"given":"Sanjay","family":"Chhibber","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Panjab University, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5431-8183","authenticated-orcid":false,"given":"Kusum","family":"Harjai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Panjab University, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Catheter-associated urinary tract infections (CAUTI)","author":"CDC","year":"2015","unstructured":"CDC. 2015. 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