{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T12:50:00Z","timestamp":1759063800979,"version":"3.41.2"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2020,3,24]],"date-time":"2020-03-24T00:00:00Z","timestamp":1585008000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["436202"],"award-info":[{"award-number":["436202"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Gouvernement du Canada | Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["148361"],"award-info":[{"award-number":["148361"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,3,24]]},"abstract":"<jats:p>\n            Herein, we phenotypically and enzymatically characterize the theoretical mutation Q579I in helix K and the already described clinical mutation K805Q in helix P of cytomegalovirus DNA polymerase for susceptibility to foscarnet. Q579I and K805Q recombinant viruses were hypersusceptible to foscarnet (respective mean 50% effective concentrations [EC\n            <jats:sub>50<\/jats:sub>\n            ] of 0.12- and 0.19-fold that of the wild type). Three-dimensional modeling analysis suggested that both mutations favor the closed conformation of the enzyme to which foscarnet binds with a higher affinity.\n          <\/jats:p>","DOI":"10.1128\/aac.01910-19","type":"journal-article","created":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T09:13:31Z","timestamp":1580375611000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Hypersusceptibility of Human Cytomegalovirus to Foscarnet Induced by Mutations in Helices K and P of the Viral DNA Polymerase"],"prefix":"10.1128","volume":"64","author":[{"given":"Karima","family":"Zarrouk","sequence":"first","affiliation":[{"name":"Research Center in Infectious Diseases, CHU de Qu\u00e9bec-Laval University, Quebec City, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Van Dung","family":"Pham","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology, and Bioinformatics, PROTEO, Laval University, Quebec City, Quebec, Canada"},{"name":"Institute of Integrative and Systems Biology, Laval University, Quebec City, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jocelyne","family":"Piret","sequence":"additional","affiliation":[{"name":"Research Center in Infectious Diseases, CHU de Qu\u00e9bec-Laval University, Quebec City, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology, and Bioinformatics, PROTEO, Laval University, Quebec City, Quebec, Canada"},{"name":"Institute of Integrative and Systems Biology, Laval University, Quebec City, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Boivin","sequence":"additional","affiliation":[{"name":"Research Center in Infectious Diseases, CHU de Qu\u00e9bec-Laval 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