{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T13:42:38Z","timestamp":1774705358459,"version":"3.50.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K23AI103044"],"award-info":[{"award-number":["K23AI103044"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000061","name":"HHS | NIH | Fogarty International Center","doi-asserted-by":"publisher","award":["D43TW007124"],"award-info":[{"award-number":["D43TW007124"]}],"id":[{"id":"10.13039\/100000061","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"HHS | NIH | National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR000454"],"award-info":[{"award-number":["UL1TR000454"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The country of Georgia has a high burden of multi- and extensively drug-resistant tuberculosis (XDR-TB). To evaluate whether mutations in\n            <jats:italic>gyrB<\/jats:italic>\n            and\n            <jats:italic>eis<\/jats:italic>\n            genes increased the sensitivity of detection of phenotypic resistance to ofloxacin and kanamycin or capreomycin compared to use of the first-generation MTBDR\n            <jats:italic>sl<\/jats:italic>\n            assay alone, which tests for mutations in\n            <jats:italic>gyrA<\/jats:italic>\n            and\n            <jats:italic>rrs<\/jats:italic>\n            genes, a retrospective study of stored\n            <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n            isolates was performed. All isolates underwent DNA sequencing of resistance-determining regions. Among 112\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            isolates with DNA extraction data, targeted sequencing was successfully performed for each gene as follows: for\n            <jats:italic>gyrA<\/jats:italic>\n            , 98% sensitivity; for\n            <jats:italic>gyrB<\/jats:italic>\n            , 96%; for\n            <jats:italic>rrs<\/jats:italic>\n            , 93%; for the\n            <jats:italic>eis<\/jats:italic>\n            gene and its promoter, 93%. The specificity and hence the positive predictive value of\n            <jats:italic>gyrA<\/jats:italic>\n            and\n            <jats:italic>gyrB<\/jats:italic>\n            mutations for detecting ofloxacin resistance were 100%. The addition of\n            <jats:italic>gyrB<\/jats:italic>\n            mutations increased the sensitivity of phenotypic ofloxacin resistance detection by 13% (75% to 88%). All\n            <jats:italic>rrs<\/jats:italic>\n            resistance-conferring mutations were A1401G, and this mutation had low sensitivity (40% and 18%) and high specificity (95% and 100%) in predicting phenotypic capreomycin and kanamycin resistance, respectively. The\n            <jats:italic>eis<\/jats:italic>\n            C-14T mutation increased the sensitivity of phenotypic kanamycin resistance detection by 9% (18% to 27%) and was found solely in kanamycin phenotypic resistance isolates. Our data showed that the inclusion of\n            <jats:italic>eis<\/jats:italic>\n            C-14T and\n            <jats:italic>gyrB<\/jats:italic>\n            mutations in addition to\n            <jats:italic>rrs<\/jats:italic>\n            and\n            <jats:italic>gyrA<\/jats:italic>\n            mutations improves the sensitivity of detection of phenotypic ofloxacin and kanamycin resistance, respectively.\n          <\/jats:p>","DOI":"10.1128\/aac.01921-16","type":"journal-article","created":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T00:30:28Z","timestamp":1497918628000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Impact of\n            <i>gyrB<\/i>\n            and\n            <i>eis<\/i>\n            Mutations in Improving Detection of Second-Line-Drug Resistance among Mycobacterium tuberculosis Isolates from Georgia"],"prefix":"10.1128","volume":"61","author":[{"given":"N.","family":"Bablishvili","sequence":"first","affiliation":[{"name":"National Center for Tuberculosis and Lung Diseases, Tbilisi, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Tukvadze","sequence":"additional","affiliation":[{"name":"National Center for Tuberculosis and Lung Diseases, Tbilisi, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Shashkina","sequence":"additional","affiliation":[{"name":"Public Health Research Institute, Rutgers University, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Mathema","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. R.","family":"Gandhi","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA"},{"name":"Departments of Epidemiology and Global Health, Emory Rollins School of Public Health, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. M.","family":"Blumberg","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. R.","family":"Kempker","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Global tuberculosis report","author":"WHO","year":"2015","unstructured":"WHO. 2015. Global tuberculosis report. WHO\/HTM\/TB\/2015.22. WHO, Geneva, Switzerland."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)00251-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(14)70031-1"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu619"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02289-08"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.11.101436"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031563"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.5588\/ijtld.14.0867"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu631"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD010705.pub2"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.5588\/ijtld.13.0468"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks178"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907925106"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04058-14"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02701-13"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01257-15"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv279"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039754"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02775-15"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033275"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmyco.2015.09.001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00851-09"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04438-14"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00662-15"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00472-13"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01602-13"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.5588\/ijtld.15.0221"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-10-2967"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01921-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01921-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T20:52:56Z","timestamp":1645476776000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01921-16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9]]},"references-count":28,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1128\/AAC.01921-16"],"URL":"https:\/\/doi.org\/10.1128\/aac.01921-16","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9]]},"assertion":[{"value":"2016-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-04-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01921-16"}}