{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:19:25Z","timestamp":1785410365442,"version":"3.56.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:00:00Z","timestamp":1748995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2025,6,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The emergence of\n                      <jats:italic toggle=\"yes\">Plasmodium falciparum<\/jats:italic>\n                      parasites partially resistant to artemisinins (ART-R) poses a significant threat to recent gains in malaria control. ART-R has been associated with PfKelch13 (K13) mutations, which differ in fitness costs. This study investigates the gametocyte production and transmission fitness of African and Asian\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      isolates with different K13 genotypes across multiple mosquito species. We tested three ART-sensitive (ART-S) isolates (NF54, NF135, and NF180) and three ART-R isolates (ARN1G, 3815, and PAT-023) for sexual conversion and transmission to\n                      <jats:italic toggle=\"yes\">Anopheles stephensi, An. gambiae,<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">An. coluzzii<\/jats:italic>\n                      . ART-R levels were quantified\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      using the Ring-stage Survival Assay (RSA), and the transmission-reducing effects of dihydroartemisinin (DHA) on mature gametocytes were assessed. Results showed that ART-S parasite lines consistently produced gametocytes and transmitted effectively in all three mosquito species. ART-R isolates showed variability: ARN1G maintained high transmission levels, whereas 3815 showed limited transmission potential despite higher sporozoite loads in\n                      <jats:italic toggle=\"yes\">An. coluzzii<\/jats:italic>\n                      . The African ART-R isolate PAT-023 demonstrated low gametocyte commitment but was transmitted efficiently in both\n                      <jats:italic toggle=\"yes\">An. gambiae<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">An. coluzzii<\/jats:italic>\n                      . DHA exposure reduced mosquito infectivity for all isolates, regardless of K13 genotype. These findings, based on a limited number of field isolates, suggest that ART-R parasites remain transmissible across different\n                      <jats:italic toggle=\"yes\">Anopheles<\/jats:italic>\n                      species. However, ART-R does not appear to confer a direct transmission advantage. This study highlights the complexity of ART-R dynamics and underscores the need for further research to inform malaria control strategies in regions where ART-R parasites are circulating.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aac.01930-24","type":"journal-article","created":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T09:04:12Z","timestamp":1745312652000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Gametocyte production and transmission fitness of African and Asian\n                    <i>Plasmodium falciparum<\/i>\n                    isolates with differential susceptibility to artemisinins"],"prefix":"10.1128","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3750-1135","authenticated-orcid":true,"given":"Nicholas I.","family":"Proellochs","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Radboud 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