{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T21:20:23Z","timestamp":1789507223933,"version":"build-2803163510"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC31500055"],"award-info":[{"award-number":["NSFC31500055"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20140898"],"award-info":[{"award-number":["BK20140898"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007166","name":"Jiangsu Provincial Department of Education","doi-asserted-by":"publisher","award":["14KJB330004"],"award-info":[{"award-number":["14KJB330004"]}],"id":[{"id":"10.13039\/501100007166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Antifungal treatment is often ineffectual, partly because of biofilm formation. In this study, by using a combined forward and reverse genetic strategy, we identified that nucleus-localized AfSsn3 and its partner AfSsn8, which constitute a Cdk8-cyclin pair, are required for azole resistance in\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            . Deletion of\n            <jats:italic>Afssn3<\/jats:italic>\n            led to increased absorption and utilization of glucose and amino acids. Interestingly, absorption and utilization of glucose accelerated the extracellular polysaccharide formation, while utilization of the amino acids serine, threonine, and glycine increased sphingolipid pathway intermediate accumulation. In addition, the absence of\n            <jats:italic>Afssn3<\/jats:italic>\n            induced the activity of the efflux pump proteins. These factors indicate the mature biofilm is responsible for the major mechanisms of\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            resistance to azoles in the \u0394\n            <jats:italic>Afssn3<\/jats:italic>\n            mutant. Collectively, the loss of\n            <jats:italic>Afssn3<\/jats:italic>\n            led to two \u201cbarrier\u201d layers between the intracellular and extracellular spaces, which consequently decreased drug penetration into the cell.\n          <\/jats:p>","DOI":"10.1128\/aac.01978-17","type":"journal-article","created":{"date-parts":[[2018,1,5]],"date-time":"2018-01-05T20:34:34Z","timestamp":1515184474000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Aspergillus fumigatus\n            <i>Afssn3-Afssn8<\/i>\n            Pair Reverse Regulates Azole Resistance by Conferring Extracellular Polysaccharide, Sphingolipid Pathway Intermediates, and Efflux Pumps to Biofilm"],"prefix":"10.1128","volume":"62","author":[{"given":"Nanbiao","family":"Long","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Shaoyang University, Shaoyang, 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