{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T09:17:53Z","timestamp":1791883073893,"version":"4.3.4"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Fondo de Investigaci\u00f3n Sanitaria","award":["PI14\/00740"],"award-info":[{"award-number":["PI14\/00740"]}]},{"name":"Fondo de Investigaci\u00f3n Sanitaria","award":["MSI15\/00115"],"award-info":[{"award-number":["MSI15\/00115"]}]},{"name":"CM-SANTANDER","award":["GR3\/2014"],"award-info":[{"award-number":["GR3\/2014"]}]},{"name":"Spanish Network for Research in Infectious Diseases","award":["RD12\/0015"],"award-info":[{"award-number":["RD12\/0015"]}]},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1on","award":["II-Predoc-2016-IISGM"],"award-info":[{"award-number":["II-Predoc-2016-IISGM"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We report the mutant prevention concentration (MPC) and mutant selection window (MSW) for micafungin and anidulafungin administered to treat\n                    <jats:named-content content-type=\"genus-species\">Candida glabrata<\/jats:named-content>\n                    . We also determine the mutation frequency. We studied 20 echinocandin-susceptible, fluconazole-intermediate, and\n                    <jats:italic>FKS<\/jats:italic>\n                    wild-type\n                    <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n                    isolates. Adjusted inocula were stroked directly onto Sabouraud agar plates containing different concentrations of micafungin or anidulafungin and visually inspected daily for up to 5 days of incubation. Individual colonies growing on the plates containing echinocandins at 1 mg\/liter were selected for antifungal susceptibility testing. The\n                    <jats:italic>FKS<\/jats:italic>\n                    genes of the resulting individual phenotypically resistant colonies were sequenced, and the MPC, MSW, and mutation frequency were determined. Biofilm was quantified, and the growth kinetics and virulence (\n                    <jats:named-content content-type=\"genus-species\">Galleria mellonella<\/jats:named-content>\n                    model) of the resulting individual\n                    <jats:italic>FKS<\/jats:italic>\n                    mutant colonies were studied. For micafungin and anidulafungin, we found similar results for the MPC (0.06 to 2 mg\/liter and 0.25 to 2 mg\/liter, respectively), MSW (0.015 to 2 mg\/liter for both echinocandins), and mutation frequency (3.7 \u00d7 10\n                    <jats:sup>\u22128<\/jats:sup>\n                    and 2.8 \u00d7 10\n                    <jats:sup>\u22128<\/jats:sup>\n                    , respectively). A total of 12 isolates were able to grow at 1 mg\/liter on echinocandin-containing plates, yielding a total of 32 phenotypically resistant colonies; however,\n                    <jats:italic>FKS2<\/jats:italic>\n                    mutations (\u0394F658, S663P, W715L, and E655A) were observed only in 21 colonies. We did not find differences in biofilm formation, the kinetic parameters studied, or the median survival of larvae infected by wild-type isolates and the resulting individual\n                    <jats:italic>FKS2<\/jats:italic>\n                    mutant colonies. Echinocandin concentrations lower than 2 mg\/liter can lead to selection of resistance mutations in\n                    <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n                    isolates\n                    <jats:italic>in vitro<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/aac.01982-17","type":"journal-article","created":{"date-parts":[[2018,1,8]],"date-time":"2018-01-08T07:05:36Z","timestamp":1515395136000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Mutant Prevention Concentration and Mutant Selection Window of Micafungin and Anidulafungin in Clinical Candida glabrata Isolates"],"prefix":"10.1128","volume":"62","author":[{"given":"Mar\u00eda \u00c1ngeles","family":"Bordallo-Cardona","sequence":"first","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio 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