{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:58:32Z","timestamp":1788307112389,"version":"build-2803163510"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Class D \u03b2-lactamases capable of hydrolyzing last-resort carbapenem antibiotics represent a major challenge for treatment of bacterial infections. Wide dissemination of these enzymes in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter baumannii<\/jats:named-content>\n            elevated this pathogen to the category of most deadly and difficult to treat. We present here the structure of the OXA-58 \u03b2-lactamase, a major class D carbapenemase of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n            , determined to 1.30-\u212b resolution. Unlike two other\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter<\/jats:named-content>\n            carbapenemases, OXA23 and OXA-24, the OXA-58 enzyme lacks the characteristic hydrophobic bridge over the active site, despite conservation of the residues which participate in its formation. The active-site residues in OXA-58 are spatially conserved in comparison to those in other class D \u03b2-lactamases. Lys86, which activates water molecules during the acylation and deacylation steps, is fully carboxylated in the OXA-58 structure. In the absence of a substrate, a water molecule is observed in the active site of the enzyme and is positioned in the pocket that is usually occupied by the 6\u03b1-hydroxyethyl moiety of carbapenems. A water molecule in this location would efficiently deacylate good substrates, such as the penicillins, but in the case of carbapenems, it would be expelled by the 6\u03b1-hydroxyethyl moiety of the antibiotics and a water from the surrounding medium would find its way to the vicinity of the carboxylated Lys86 to perform deacylation. Subtle differences in the position of this water in the acyl-enzyme complexes of class D \u03b2-lactamases could ultimately be responsible for differences in the catalytic efficiencies of these enzymes against last-resort carbapenem antibiotics.\n          <\/jats:p>","DOI":"10.1128\/aac.01983-13","type":"journal-article","created":{"date-parts":[[2014,1,28]],"date-time":"2014-01-28T05:28:10Z","timestamp":1390886890000},"page":"2135-2143","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Crystal Structure of Carbapenemase OXA-58 from Acinetobacter baumannii"],"prefix":"10.1128","volume":"58","author":[{"given":"Clyde A.","family":"Smith","sequence":"first","affiliation":[{"name":"Stanford Synchrotron Radiation Lightsource, Stanford University, Menlo Park, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuno Tiago","family":"Antunes","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Toth","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergei B.","family":"Vakulenko","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00058-07"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr290"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp428"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/652152"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2011.06.011"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.09.014"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01464-06"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2006.01456.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.6.1556-1561.2000"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.1.196-199.2000"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.10.4174-4179.2005"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0924-8579(93)90045-7"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.1.202-208.2005"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01512-08"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00001-07"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607557104"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2009.04.010"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2010.12.042"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00762-13"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2013.07.015"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0013881"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/79688"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4805"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01517-09"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1701"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(68)90205-2"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889893005588"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910045749"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444905036693"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1107\/S090744499801405X"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904019158"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444904026460"},{"key":"e_1_3_3_36_2","volume-title":"The PyMOL molecular graphics system","author":"DeLano WL","year":"2002","unstructured":"DeLanoWL. 2002. The PyMOL molecular graphics system. Schr\u00f6dinger, LLC, San Carlos, CA."},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.0224303"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi901690r"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi972127f"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241442898"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2790213"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340160406"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/359700a0"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444907036955"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0049035"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.458315"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzr041"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki482"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00541a008"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.280115"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1218231"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889892009944"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01983-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.01983-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T22:43:02Z","timestamp":1645483382000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.01983-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":51,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1128\/AAC.01983-13"],"URL":"https:\/\/doi.org\/10.1128\/aac.01983-13","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4]]},"assertion":[{"value":"2013-09-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}