{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:13:51Z","timestamp":1790439231227,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003825","name":"Hungarian Academy of Sciences","doi-asserted-by":"crossref","award":["LP2012-22"],"award-info":[{"award-number":["LP2012-22"]}],"id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The molecular mechanisms of resistance to fluoroquinolones, tetracyclines, an aminocyclitol, macrolides, a lincosamide, a phenicol, and pleuromutilins were investigated in\n                    <jats:named-content content-type=\"genus-species\">Mycoplasma bovis<\/jats:named-content>\n                    . For the identification of mutations responsible for the high MICs of certain antibiotics, whole-genome sequencing of 35\n                    <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n                    field isolates and 36 laboratory-derived antibiotic-resistant mutants was performed.\n                    <jats:italic>In vitro<\/jats:italic>\n                    resistant mutants were selected by serial passages of\n                    <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n                    in broth medium containing subinhibitory concentrations of the antibiotics. Mutations associated with high fluoroquinolones MICs were found at positions 244 to 260 and at positions 232 to 250 (according to\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    numbering) of the quinolone resistance-determining regions of the\n                    <jats:italic>gyrA<\/jats:italic>\n                    and\n                    <jats:italic>parC<\/jats:italic>\n                    genes, respectively. Alterations related to elevated tetracycline MICs were described at positions 962 to 967, 1058, 1195, 1196, and 1199 of genes encoding the 16S rRNA and forming the primary tetracycline binding site. Single transversion at position 1192 of the\n                    <jats:italic>rrs1<\/jats:italic>\n                    gene resulted in a spectinomycin MIC of 256 \u03bcg\/ml. Mutations responsible for high macrolide, lincomycin, florfenicol, and pleuromutilin antibiotic MICs were identified in genes encoding 23S rRNA. Understanding antibiotic resistance mechanisms is an important tool for future developments of genetic-based diagnostic assays for the rapid detection of resistant\n                    <jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>\n                    strains.\n                  <\/jats:p>","DOI":"10.1128\/aac.01983-16","type":"journal-article","created":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T21:31:07Z","timestamp":1480368667000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Mutations Associated with Decreased Susceptibility to Seven Antimicrobial Families in Field and Laboratory-Derived Mycoplasma bovis Strains"],"prefix":"10.1128","volume":"61","author":[{"given":"Kinga M.","family":"Sulyok","sequence":"first","affiliation":[{"name":"Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, 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