{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:04:52Z","timestamp":1790719492778,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We investigated the efficacies of cefotaxime (CTX) and amoxicillin (AMX)-clavulanate (CLA) (AMC) against extended-spectrum-\u03b2-lactamase (ESBL)-producing\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            <jats:italic>in vitro<\/jats:italic>\n            and in a murine model of urinary tract infection (UTI). MICs, the checkerboard dilution method, and time-kill curves were used to explore the\n            <jats:italic>in vitro<\/jats:italic>\n            synergism between cefotaxime and amoxicillin-clavulanate against two isogenic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            strains\u2014CFT073-RR and its transconjugant, CFT073-RR Tc\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            \u2014harboring a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            plasmid and a\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>OXA-1<\/jats:sub>\n            plasmid. For\n            <jats:italic>in vivo<\/jats:italic>\n            experiments, mice were separately infected with each strain and treated with cefotaxime, amoxicillin, and clavulanate, alone or in combination, or imipenem, using therapeutic regimens reproducing time of free-drug concentrations above the MIC (\n            <jats:italic>fT<\/jats:italic>\n            \u2265MIC) values close to that obtained in humans. MICs of amoxicillin, cefotaxime, and imipenem were 4\/&gt;1,024, 0.125\/1,024, and 0.5\/0.5 mg\/liter, for CFT073-RR and CFT073-RR Tc\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            , respectively. The addition of 2 mg\/liter of clavulanate (CLA) restored the susceptibility of CFT073-RR Tc\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            to CTX (MICs of the CTX-CLA combination, 0.125 mg\/liter). The checkerboard dilution method and time-kill curves confirmed an\n            <jats:italic>in vitro<\/jats:italic>\n            synergy between amoxicillin-clavulanate and cefotaxime against CFT073-RR Tc\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>CTX-M-15<\/jats:sub>\n            .\n            <jats:italic>In vivo<\/jats:italic>\n            , this antibiotic combination was similarly active against both strains and as effective as imipenem. In conclusion, the cefotaxime and amoxicillin-clavulanate combination appear to be an effective, easy, and already available alternative to carbapenems for the treatment of UTI due to CTX-M-producing\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. coli<\/jats:named-content>\n            strains.\n          <\/jats:p>","DOI":"10.1128\/aac.02018-15","type":"journal-article","created":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T01:35:21Z","timestamp":1446514521000},"page":"424-430","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Cefotaxime and Amoxicillin-Clavulanate Synergism against Extended-Spectrum-\u03b2-Lactamase-Producing\n            <i>Escherichia coli<\/i>\n            in a Murine Model of Urinary Tract Infection"],"prefix":"10.1128","volume":"60","author":[{"given":"B.","family":"Rossi","sequence":"first","affiliation":[{"name":"INSERM UMR1137, IAME, Paris, France"},{"name":"AP-HP, H\u00f4pital Beaujon, Service de M\u00e9decine Interne, Clichy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. F.","family":"Soubirou","sequence":"additional","affiliation":[{"name":"INSERM UMR1137, IAME, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Chau","sequence":"additional","affiliation":[{"name":"INSERM UMR1137, IAME, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Massias","sequence":"additional","affiliation":[{"name":"INSERM UMR1137, IAME, Paris, France"},{"name":"AP-HP, H\u00f4pital Bichat, Laboratoire de Toxicologie-Pharmacocin\u00e9tique, Service de Pharmacie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Dion","sequence":"additional","affiliation":[{"name":"INSERM UMR1137, IAME, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Lepeule","sequence":"additional","affiliation":[{"name":"INSERM UMR1137, IAME, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Fantin","sequence":"additional","affiliation":[{"name":"INSERM UMR1137, IAME, Paris, France"},{"name":"AP-HP, H\u00f4pital Beaujon, Service de M\u00e9decine Interne, Clichy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lefort","sequence":"additional","affiliation":[{"name":"INSERM UMR1137, IAME, Paris, France"},{"name":"AP-HP, H\u00f4pital Beaujon, Service de M\u00e9decine Interne, Clichy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.08.011"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01823-12"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir790"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks301"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06001-11"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.medmal.2013.01.006"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"S189","DOI":"10.1111\/j.1469-0691.2007.01858.x","article-title":"Orthodox and unorthodox clavulanate combinations against extended-spectrum beta-lactamase producers","volume":"14","author":"Livermore DM","year":"2008","unstructured":"Livermore DM, Hope R, Mushtaq S, Warner M. 2008. 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