{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T09:47:24Z","timestamp":1787478444611,"version":"build-2736575974"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bacterial infections are known to cause severe health-threatening conditions, including sepsis. All attempts to get this disease under control failed in the past, and especially in times of increasing antibiotic resistance, this leads to one of the most urgent medical challenges of our times. We designed a peptide to bind with high affinity to endotoxins, one of the most potent pathogenicity factors involved in triggering sepsis. The peptide Pep19-2.5 reveals high endotoxin neutralization efficiency\n            <jats:italic>in vitro<\/jats:italic>\n            , and here, we demonstrate its antiseptic\/anti-inflammatory effects\n            <jats:italic>in vivo<\/jats:italic>\n            in the mouse models of endotoxemia, bacteremia, and cecal ligation and puncture, as well as in an\n            <jats:italic>ex vivo<\/jats:italic>\n            model of human tissue. Furthermore, we show that Pep19-2.5 can bind and neutralize not only endotoxins but also other bacterial pathogenicity factors, such as those from the Gram-positive bacterium\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Staphylococcus aureus<\/jats:named-content>\n            . This broad neutralization efficiency and the additive action of the peptide with common antibiotics makes it an exceptionally appropriate drug candidate against bacterial sepsis and also offers multiple other medication opportunities.\n          <\/jats:p>","DOI":"10.1128\/aac.02066-12","type":"journal-article","created":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T04:53:42Z","timestamp":1358225622000},"page":"1480-1487","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["Preclinical Investigations Reveal the Broad-Spectrum Neutralizing Activity of Peptide Pep19-2.5 on Bacterial Pathogenicity Factors"],"prefix":"10.1128","volume":"57","author":[{"given":"Lena","family":"Heinbockel","sequence":"first","affiliation":[{"name":"Divisions of Biophysics, Research Center Borstel, Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"S\u00e1nchez-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, University of Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo","family":"Martinez de Tejada","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology, University of Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"D\u00f6mming","sequence":"additional","affiliation":[{"name":"Department of Intensive Care, University Hospital Aachen, Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julius","family":"Brandenburg","sequence":"additional","affiliation":[{"name":"Divisions of Biophysics, Research Center Borstel, Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yani","family":"Kaconis","sequence":"additional","affiliation":[{"name":"Divisions of Biophysics, Research Center Borstel, Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Hornef","sequence":"additional","affiliation":[{"name":"Hannover Medical School, Institute for Medical Microbiology and Hospital Epidemiology, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Dupont","sequence":"additional","affiliation":[{"name":"Hannover Medical School, Institute for Medical Microbiology and Hospital Epidemiology, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Marwitz","sequence":"additional","affiliation":[{"name":"Pathology, Research Center Borstel, Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"Goldmann","sequence":"additional","affiliation":[{"name":"Pathology, Research Center Borstel, Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Ernst","sequence":"additional","affiliation":[{"name":"Divisions of Biophysics, Research Center Borstel, Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Gutsmann","sequence":"additional","affiliation":[{"name":"Divisions of Biophysics, Research Center Borstel, Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Sch\u00fcrholz","sequence":"additional","affiliation":[{"name":"Department of Intensive Care, University Hospital Aachen, Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Brandenburg","sequence":"additional","affiliation":[{"name":"Divisions of Biophysics, Research Center Borstel, Borstel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200107000-00002"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000277043.85378.C1"},{"key":"e_1_3_3_4_2","first-page":"692","article-title":"The Surviving Sepsis Campaign guidelines: should we follow?","volume":"13","author":"Singer P","year":"2011","unstructured":"SingerP CohenJD. 2011. 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