{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:08:13Z","timestamp":1791288493209,"version":"4.3.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI132627"],"award-info":[{"award-number":["R01AI132627"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R56AI102503-01A1"],"award-info":[{"award-number":["1R56AI102503-01A1"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Invasive methicillin-resistant\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            (MRSA) treated with vancomycin (VAN) is associated with reduced VAN susceptibility and treatment failure. VAN combination therapy is one strategy to improve response, but comprehensive assessments of combinations to prevent resistance are limited. This study identifies optimal combinations to prevent the emergence of VAN-intermediate\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            (VISA). Two standard MRSA and two heterogeneous VISA (hVISA) strains were exposed for 28 days\n            <jats:italic>in vitro<\/jats:italic>\n            to VAN alone, VAN with cefazolin (CFZ), fosfomycin, gentamicin, meropenem, rifampin, piperacillin-tazobactam (TZP), or trimethoprim-sulfamethoxazole. In addition to VAN susceptibility testing, cell wall thickness (CWT), carotenoid content, and membrane fluidity were determined for Mu3. VAN plus any \u03b2-lactam limited the VAN MIC increase to 1 to 4 mg\/liter throughout the 28-day exposure, with CFZ and TZP being the most effective agents (VAN MIC = 1 to 2 mg\/liter). Similar MIC trends occurred with the lipo-\/glycopeptide agents daptomycin and telavancin, where \u03b2-lactam combinations with VAN prevented MIC increases to these agents as well. Combinations with non-\u03b2-lactams were ineffective in preventing VAN MIC increases with VAN MICs of 4 to 16 mg\/liter emerging during weeks 2 to 4 of treatment. VAN plus \u03b2-lactam decreased CWT significantly, whereas VAN plus other antibiotics significantly increased the CWT. No correlation was observed between carotenoid content or membrane fluidity and antibiotic exposure. Only the combination exposures of VAN plus \u03b2-lactam suppress the development of VISA. Rational selection of VAN plus \u03b2-lactam should be further explored as a long-term combination treatment of MRSA infections due to their ability to suppress VAN resistance.\n          <\/jats:p>","DOI":"10.1128\/aac.02100-17","type":"journal-article","created":{"date-parts":[[2017,11,20]],"date-time":"2017-11-20T17:10:25Z","timestamp":1511197825000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Combination Antibiotic Exposure Selectively Alters the Development of Vancomycin Intermediate Resistance in Staphylococcus aureus"],"prefix":"10.1128","volume":"62","author":[{"given":"Xuting","family":"Zheng","sequence":"first","affiliation":[{"name":"Division of Infectious Disease, The First Hospital of China Medical University, Shenyang, People's Republic of China"},{"name":"School of Pharmacy, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D.","family":"Berti","sequence":"additional","affiliation":[{"name":"School of Pharmacy, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue","family":"McCrone","sequence":"additional","affiliation":[{"name":"School of Pharmacy, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Roch","sequence":"additional","affiliation":[{"name":"Department of Pathology and Genomic Medicine, Houston Methodist Research Institute, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana E.","family":"Rosato","sequence":"additional","affiliation":[{"name":"Department of Pathology and Genomic Medicine, Houston Methodist Research Institute, Houston, Texas, 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