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Previous work implicated the\n            <jats:italic>tssC1<\/jats:italic>\n            gene in\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            biofilm-specific antibiotic resistance, and\n            <jats:italic>tssC1<\/jats:italic>\n            is preferentially expressed in biofilms compared to planktonic cells. Using a DNA-dependent protein pulldown approach, we discovered that PA3225, an uncharacterized LysR-type transcriptional regulator, specifically bound to the\n            <jats:italic>tssABC1<\/jats:italic>\n            upstream regulatory region. The deletion of PA3225 led to a 2-fold decrease in\n            <jats:italic>tssA1<\/jats:italic>\n            expression levels in planktonic cells compared to the wild type, and\n            <jats:italic>tssA1<\/jats:italic>\n            expression was slightly reduced in \u0394PA3225 biofilms compared to wild-type biofilms. Intriguingly, further investigations revealed that the \u0394PA3225 mutant was less susceptible to multiple, structurally unrelated antibiotics with various mechanisms of action when grown planktonically. The \u0394PA3225 mutant was additionally more resistant to ciprofloxacin when grown in a biofilm. The decreased antibiotic susceptibility of the \u0394PA3225 strain was linked to the transcriptional upregulation of the MexAB-OprM efflux pump. By using transcriptome sequencing (RNA-seq), other PA3225-regulated genes were identified, and the products of these genes, such as the putative ABC transporter PA3228, may also contribute to antibiotic resistance.\n          <\/jats:p>","DOI":"10.1128\/aac.02114-16","type":"journal-article","created":{"date-parts":[[2017,6,6]],"date-time":"2017-06-06T00:30:26Z","timestamp":1496709026000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["PA3225 Is a Transcriptional Repressor of Antibiotic Resistance Mechanisms in Pseudomonas aeruginosa"],"prefix":"10.1128","volume":"61","author":[{"given":"Clayton W.","family":"Hall","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of 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