{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:20:46Z","timestamp":1790634046943,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Prof. Tsuei-Chu Mong Scholarship","award":["30219003"],"award-info":[{"award-number":["30219003"]}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["104-2320-B-010-023-MY3"],"award-info":[{"award-number":["104-2320-B-010-023-MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Overexpression of resistance-nodulation-division (RND)-type efflux pumps is an important mechanism for bacteria to combat antimicrobials. RND efflux pumps are also critical for bacterial physiology, such as oxidative stress tolerance.\n            <jats:named-content content-type=\"genus-species\">Stenotrophomonas maltophilia<\/jats:named-content>\n            , a multidrug-resistant opportunistic pathogen, harbors eight RND-type efflux pump operons. Of these, the\n            <jats:italic>smeU1VWU2X<\/jats:italic>\n            operon is unique for its possession of two additional genes,\n            <jats:italic>smeU1<\/jats:italic>\n            and\n            <jats:italic>smeU2<\/jats:italic>\n            , which encode proteins of the short-chain dehydrogenase\/reductase (SDR) family. Overexpression of the SmeVWX pump is known to contribute to the acquired resistance to chloramphenicol, quinolone, and tetracycline; however, SmeU1 and SmeU2 are little involved in this phenotype. In the study described in this article, we further linked the\n            <jats:italic>smeU1VWU2X<\/jats:italic>\n            operon to oxidative stress alleviation and sulfamethoxazole-trimethoprim (SXT)-resistant mutant occurrence. The\n            <jats:italic>smeU1VWU2X<\/jats:italic>\n            operon was inducibly expressed upon challenge with menadione (MD), plumbagin (PL), and hydrogen peroxide (H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            ), as verified by the use of the chromosomal\n            <jats:italic>smeU1VWU2X-xylE<\/jats:italic>\n            transcriptional fusion construct and quantitative real-time PCR (qRT-PCR). The MD-mediated\n            <jats:italic>smeU1VWU2X<\/jats:italic>\n            upexpression was totally dependent on SoxR and partially relied on SmeRv but was less relevant to OxyR. SmeRv, but not SoxR and OxyR, played a regulatory role in the H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            -mediated\n            <jats:italic>smeU1VWU2X<\/jats:italic>\n            upexpression. The significance of\n            <jats:italic>smeU1VWU2X<\/jats:italic>\n            upexpression was investigated with respect to oxidative stress alleviation and SXT-resistant mutant occurrence. Overexpression of the\n            <jats:italic>smeU1VWU2X<\/jats:italic>\n            operon contributed to the alleviation of MD-mediated oxidative stress. Of the encoded proteins, the SmeVWX pump and SmeU2, rather than SmeU1, participated in MD tolerance. Furthermore, we also demonstrated that the MD-mediated expression of the\n            <jats:italic>smeU1VWU2X<\/jats:italic>\n            operon decreased the SXT resistance frequency when\n            <jats:named-content content-type=\"genus-species\">S. maltophilia<\/jats:named-content>\n            was grown in a reactive oxygen species (ROS)-rich environment.\n          <\/jats:p>","DOI":"10.1128\/aac.02114-17","type":"journal-article","created":{"date-parts":[[2017,12,4]],"date-time":"2017-12-04T16:44:57Z","timestamp":1512405897000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Role of\n            <i>smeU1VWU2X<\/i>\n            Operon in Alleviation of Oxidative Stresses and Occurrence of Sulfamethoxazole-Trimethoprim-Resistant Mutants in Stenotrophomonas maltophilia"],"prefix":"10.1128","volume":"62","author":[{"given":"Chao-Jung","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsu-Ting","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Tsung","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan"},{"name":"School of Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Wei","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Hua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei, Taiwan"},{"name":"School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuey-Ching","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"3","article-title":"Oxidative stress in bacteria and protein damage by reactive oxygen species","volume":"3","author":"Cabiscol E","year":"2000","unstructured":"Cabiscol E, Tamarit J, Ros J. 2000. Oxidative stress in bacteria and protein damage by reactive oxygen species. 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