{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:26:51Z","timestamp":1772162811525,"version":"3.50.1"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000854","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["RGY0066\/2016"],"award-info":[{"award-number":["RGY0066\/2016"]}],"id":[{"id":"10.13039\/501100000854","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["100993\/Z\/13\/Z"],"award-info":[{"award-number":["100993\/Z\/13\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2020,4,21]]},"abstract":"<jats:p>\n                    Advances in synthetic biology have enabled the production of a variety of compounds using bacteria as a vehicle for complex compound biosynthesis. Violacein, a naturally occurring indole pigment with antibiotic properties, can be biosynthetically engineered in\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    expressing its nonnative synthesis pathway. To explore whether this synthetic biosynthesis platform could be used for drug discovery, here we have screened bacterially derived violacein against the main causative agent of human malaria,\n                    <jats:named-content content-type=\"genus-species\">Plasmodium falciparum<\/jats:named-content>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/aac.02129-19","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T07:07:47Z","timestamp":1583305667000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["A Biosynthetic Platform for Antimalarial Drug Discovery"],"prefix":"10.1128","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1109-3727","authenticated-orcid":false,"given":"Mark D.","family":"Wilkinson","sequence":"first","affiliation":[{"name":"Department of Chemistry, Imperial College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3148-5525","authenticated-orcid":false,"given":"Hung-En","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Faculty of Medicine, Imperial College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5658-8486","authenticated-orcid":false,"given":"Paul S.","family":"Freemont","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Faculty of Medicine, Imperial College London, London, United Kingdom"},{"name":"UK Dementia Research Institute Care Research and Technology Centre, Imperial College London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0275-352X","authenticated-orcid":false,"given":"Jake","family":"Baum","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Imperial College London, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"World malaria report 2018","author":"WHO","year":"2019","unstructured":"WHO. 2019. World malaria report 2018. WHO, Geneva, Switzerland."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1403007"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.01.001"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-2040-0"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-016-1206-9"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14412"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetlet.2014.02.111"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol400654r"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00693-08"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2006.03155.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(53)91132-5"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2013.08.004"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12896-018-0428-z"},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","unstructured":"Lai H-E Obled AM Chee SM Morgan RM Sharma SV Moore SJ Polizzi KM Goss RJ Freemont PS. 2019. A GenoChemetic strategy for derivatization of the violacein natural product scaffold. bioRxiv https:\/\/doi.org\/10.1101\/202523.","DOI":"10.1101\/202523"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-011-0827-x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2008.11.008"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep15598"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-015-0452-2"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0001-706x(94)90039-6"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03666-14"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2019.111678"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1465"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3525"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3189"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032188"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.6b01109"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15160"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-017-0406-2"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.15-276618"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260403"},{"key":"e_1_3_3_32_2","doi-asserted-by":"crossref","unstructured":"Ashdown GW Dimon M Fan M Ter\u00e1n F-R Witmer K Gaboriau DCA Armstrong Z Hazard J Ando DM Baum J. 2019. A machine learning approach to define antimalarial drug action from heterogeneous cell-based screens. bioRxiv https:\/\/doi.org\/10.1101\/2019.12.19.882480.","DOI":"10.1101\/2019.12.19.882480"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02129-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02129-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T16:48:24Z","timestamp":1645462104000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.02129-19"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,21]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,4,21]]}},"alternative-id":["10.1128\/AAC.02129-19"],"URL":"https:\/\/doi.org\/10.1128\/aac.02129-19","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/814715","asserted-by":"object"}]},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,21]]},"assertion":[{"value":"2019-10-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02129-19"}}