{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:33:28Z","timestamp":1790735608607,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Although bacterial mechanisms involved in the resistance to inorganic arsenic are well understood, the molecular basis for organic arsenic resistance has not been described.\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter jejuni<\/jats:named-content>\n            , a major food-borne pathogen causing gastroenteritis in humans, is highly prevalent in poultry and is reportedly resistant to the arsenic compound roxarsone (4-hydroxy-3-nitrobenzenearsonic acid), which has been used as a feed additive in the poultry industry for growth promotion. In this study, we report the identification of a novel membrane transporter (named ArsP) that contributes to organic arsenic resistance in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter<\/jats:named-content>\n            . ArsP is predicted to be a membrane permease containing eight transmembrane helices, distinct from other known arsenic transporters. Analysis of multiple\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. jejuni<\/jats:named-content>\n            isolates from various animal species revealed that the presence of an intact\n            <jats:italic>arsP<\/jats:italic>\n            gene is associated with elevated resistance to roxarsone. In addition, inactivation of\n            <jats:italic>arsP<\/jats:italic>\n            in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. jejuni<\/jats:named-content>\n            resulted in 4- and 8-fold reductions in the MICs of roxarsone and nitarsone, respectively, compared to that for the wild-type strain. Furthermore, cloning of\n            <jats:italic>arsP<\/jats:italic>\n            into a\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. jejuni<\/jats:named-content>\n            strain lacking a functional\n            <jats:italic>arsP<\/jats:italic>\n            gene led to 16- and 64-fold increases in the MICs of roxarsone and nitarsone, respectively. Neither mutation nor overexpression of\n            <jats:italic>arsP<\/jats:italic>\n            affected the MICs of inorganic arsenic, including arsenite and arsenate, in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Campylobacter<\/jats:named-content>\n            . Moreover, acquisition of\n            <jats:italic>arsP<\/jats:italic>\n            in NCTC 11168 led to accumulation of less roxarsone than the wild-type strain lacking\n            <jats:italic>arsP<\/jats:italic>\n            . Together, these results indicate that ArsP functions as an efflux transporter specific for extrusion of organic arsenic and contributes to the resistance to these compounds in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">C. jejuni<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.02137-13","type":"journal-article","created":{"date-parts":[[2014,1,14]],"date-time":"2014-01-14T05:33:01Z","timestamp":1389677581000},"page":"2021-2029","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Identification of a Novel Membrane Transporter Mediating Resistance to Organic Arsenic in Campylobacter jejuni"],"prefix":"10.1128","volume":"58","author":[{"given":"Zhangqi","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, Iowa State 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