{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:59:06Z","timestamp":1791273546851,"version":"4.1.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Reference broth microdilution methods of\n Candida<\/jats:named-content>\n echinocandin susceptibility testing are limited by interlaboratory variability in caspofungin MICs. Recently revised Clinical and Laboratory Standards Institute (CLSI) breakpoint MICs for echinocandin nonsusceptibility may not be valid for commercial tests employed in hospital laboratories. Indeed, there are limited echinocandin susceptibility testing data from hospital laboratories. We conducted a multicenter retrospective study of 9 U.S., Australian, and New Zealand hospitals that routinely tested\n Candida<\/jats:named-content>\n bloodstream isolates for echinocandin susceptibility from 2005 to 2013. Eight hospitals used Sensititre YeastOne assays. The\n Candida<\/jats:named-content>\n spp. were\n C. albicans<\/jats:named-content>\n (\n n<\/jats:italic>\n = 1,067),\n C. glabrata<\/jats:named-content>\n (\n n<\/jats:italic>\n = 911),\n C. parapsilosis<\/jats:named-content>\n (\n n<\/jats:italic>\n = 476),\n C. tropicalis<\/jats:named-content>\n (\n n<\/jats:italic>\n = 185),\n C. krusei<\/jats:named-content>\n (\n n<\/jats:italic>\n = 104), and others (\n n<\/jats:italic>\n = 154). Resistance and intermediate rates were \u22641.4% and \u22643%, respectively, for each echinocandin against\n C. albicans<\/jats:named-content>\n ,\n C. parapsilosis<\/jats:named-content>\n , and\n C. tropicalis<\/jats:named-content>\n . Resistance rates among\n C. glabrata<\/jats:named-content>\n and\n C. krusei<\/jats:named-content>\n isolates were \u22647.5% and \u22645.6%, respectively. Caspofungin intermediate rates among\n C. glabrata<\/jats:named-content>\n and\n C. krusei<\/jats:named-content>\n isolates were 17.8% and 46.5%, respectively, compared to \u22644.3% and \u22644.4% for other echinocandins. Using CLSI breakpoints, 18% and 19% of\n C. glabrata<\/jats:named-content>\n isolates were anidulafungin susceptible\/caspofungin nonsusceptible and micafungin susceptible\/caspofungin nonsusceptible, respectively; similar discrepancies were observed for 38% and 39% of\n C. krusei<\/jats:named-content>\n isolates. If only YeastOne data were considered, interhospital modal MIC variability was low (within 2 doubling dilutions for each agent). In conclusion, YeastOne assays employed in hospitals may reduce the interlaboratory variability in caspofungin MICs against\n Candida<\/jats:named-content>\n species that are observed between reference laboratories using CLSI broth microdilution methods. The significance of classifying isolates as caspofungin intermediate and anidulafungin\/micafungin susceptible will require clarification in future studies.\n <\/jats:p>","DOI":"10.1128\/aac.02163-13","type":"journal-article","created":{"date-parts":[[2014,1,6]],"date-time":"2014-01-06T22:18:30Z","timestamp":1389046710000},"page":"1897-1906","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Real-World Experience with Echinocandin MICs against Candida Species in a Multicenter Study of Hospitals That Routinely Perform Susceptibility Testing of Bloodstream Isolates"],"prefix":"10.1128","volume":"58","author":[{"given":"Gregory A.","family":"Eschenauer","sequence":"first","affiliation":[{"name":"Department of Pharmacy and Antibiotic Management Program, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Hong","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Pittsburgh, and Antibiotic Management Program, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shmuel","family":"Shoham","sequence":"additional","affiliation":[{"name":"Department of Medicine, The Johns Hopkins Hospital, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose A.","family":"Vazquez","sequence":"additional","affiliation":[{"name":"Henry Ford Hospital, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur J.","family":"Morris","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Auckland City Hospital, Auckland, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William A.","family":"Pasculle","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine J.","family":"Kubin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, New York Presbyterian Hospital, New York, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth P.","family":"Klinker","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, UF Health Shands Hospital, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peggy L.","family":"Carver","sequence":"additional","affiliation":[{"name":"Department of Clinical, Social and Administrative Sciences, University of Michigan College of Pharmacy, and University of Michigan Health System, Ann Arbor, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly E.","family":"Hanson","sequence":"additional","affiliation":[{"name":"Department of Pathology, The University of Utah, Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Chen","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology, Westmead Hospital, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon W.","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Cleveland Clinic Foundation, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian A.","family":"Potoski","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Therapeutics, University of Pittsburgh School of Pharmacy, and Antibiotic Management Program, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lloyd G.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Antibiotic Management Program, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan K.","family":"Shields","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Pittsburgh, and Antibiotic Management Program, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelius J.","family":"Clancy","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Pittsburgh, and Antibiotic Management Program, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA"},{"name":"Department of Medicine, VA Pittsburgh Health System, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00136-13"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit136"},{"key":"e_1_3_2_4_2","volume-title":"Reference method for broth dilution antifungal susceptibility testing of yeasts; fourth informational supplement","author":"Clinical and Laboratory Standards Institute","year":"2012","unstructured":"Clinical and Laboratory Standards Institute. 2012. Reference method for broth dilution antifungal susceptibility testing of yeasts; fourth informational supplement. CLSI document M27-S4. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01935.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03647.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03880.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2011.01.004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01519-13"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00029-06"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01451-13"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.05.008"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00355-12"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01292-08"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06369-11"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/596757"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01256-09"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis021"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks482"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02163-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02163-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T17:40:50Z","timestamp":1645465250000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.02163-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1128\/AAC.02163-13"],"URL":"https:\/\/doi.org\/10.1128\/aac.02163-13","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.718229953.793492395","asserted-by":"object"}]},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4]]},"assertion":[{"value":"2013-10-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}