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The conventional method used to detect\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            is laborious. In the present study, a novel strategy was utilized to evaluate the impact of antimicrobial agents on the growth of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            and its expression of\n            <jats:italic>ompA<\/jats:italic>\n            promoter-driven green fluorescence protein (GFP). We demonstrate that this GFP reporter system gives a robust fluorescent display of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            growth in human cervical epithelial cells and, further, that GFP production directly correlates to changes in\n            <jats:italic>ompA<\/jats:italic>\n            expression following sufficient exposure to antimicrobials. Validation with azithromycin, the first-line macrolide drug used for the treatment of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            infection, highlights the advantages of this method over the traditional method because of its simplicity and versatility. The results indicate both that\n            <jats:italic>ompA<\/jats:italic>\n            is highly responsive to antimicrobials targeting the transcription and translation of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            and that there is a correlation between changing GFP levels and\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            growth. This proof-of-concept study also reveals that the\n            <jats:italic>ompA<\/jats:italic>\n            -GFP system can be easily adapted to rapidly assess antimicrobial effectiveness in a high-throughput format.\n          <\/jats:p>","DOI":"10.1128\/aac.02202-16","type":"journal-article","created":{"date-parts":[[2016,11,15]],"date-time":"2016-11-15T02:50:38Z","timestamp":1479178238000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Novel Detection Strategy To Rapidly Evaluate the Efficacy of Antichlamydial Agents"],"prefix":"10.1128","volume":"61","author":[{"given":"Yan","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Neonatology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqi","family":"Xian","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leiqiong","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiba","family":"Elaasar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lamiya","family":"Tauhid","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziyu","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis644"},{"key":"e_1_3_2_3_2","volume-title":"2015 sexually transmitted diseases treatment guidelines","author":"Centers for Disease Control and Prevention","year":"2015","unstructured":"Centers for Disease Control and Prevention. 2015. 2015 sexually transmitted diseases treatment guidelines. 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