{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:16:40Z","timestamp":1767849400343,"version":"3.49.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Metallo-\u03b2-lactamases (MBLs) have rapidly disseminated worldwide among clinically important Gram-negative bacteria and have challenged the therapeutic use of \u03b2-lactam antibiotics, particularly carbapenems. The\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>GIM-1<\/jats:sub>\n            gene, encoding one such enzyme, was first discovered in a\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Pseudomonas aeruginosa<\/jats:named-content>\n            isolate from 2002 and has more recently been reported in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterobacteriaceae<\/jats:named-content>\n            . Here, we present crystal structures of GIM-1 in the apo-zinc (metal-free), mono-zinc (where Cys221 was found to be oxidized), and di-zinc forms, providing nine independently refined views of the enzyme. GIM-1 is distinguished from related MBLs in possessing a narrower active-site groove defined by aromatic side chains (Trp228 and Tyr233) at positions normally occupied by hydrophilic residues in other MBLs. Our structures reveal considerable flexibility in two loops (loop 1, residues 60 to 66; loop 2, residues 223 to 242) adjacent to the active site, with open and closed conformations defined by alternative hydrogen-bonding patterns involving Trp228. We suggest that this capacity for rearrangement permits GIM-1 to hydrolyze a wide range of \u03b2-lactams in spite of possessing a more constrained active site. Our results highlight the structural diversity within the MBL enzyme family.\n          <\/jats:p>","DOI":"10.1128\/aac.02227-12","type":"journal-article","created":{"date-parts":[[2012,12,4]],"date-time":"2012-12-04T14:01:14Z","timestamp":1354629674000},"page":"848-854","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Crystal Structures of Pseudomonas aeruginosa GIM-1: Active-Site Plasticity in Metallo-\u03b2-Lactamases"],"prefix":"10.1128","volume":"57","author":[{"given":"Pardha Saradhi","family":"Borra","sequence":"first","affiliation":[{"name":"Research Group for Host-Microbe Interactions, Department of Medical Biology, Faculty of Health Sciences, University of Troms\u00f8, Troms\u00f8, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d8rjan","family":"Samuelsen","sequence":"additional","affiliation":[{"name":"Reference Centre for Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Troms\u00f8, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Spencer","sequence":"additional","affiliation":[{"name":"School of Cellular and Molecular Medicine, Medical Sciences Building, University of Bristol, University Walk, Bristol, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy R.","family":"Walsh","sequence":"additional","affiliation":[{"name":"Section of Medical Microbiology IIB, School of Medical Sciences, Cardiff University, Heath Park Hospital, Cardiff, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marit Sjo","family":"Lorentzen","sequence":"additional","affiliation":[{"name":"The Norwegian Structural Biology Centre (NorStruct), Department of Chemistry, University of Troms\u00f8, Troms\u00f8, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanna-Kirsti S.","family":"Leiros","sequence":"additional","affiliation":[{"name":"The Norwegian Structural Biology Centre (NorStruct), Department of Chemistry, University of Troms\u00f8, Troms\u00f8, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012272403776"},{"key":"e_1_3_3_3_2","first-page":"1","article-title":"Antibiotics: action and resistance in gram-negative bacteria","volume":"35","author":"Siu LK","year":"2002","unstructured":"SiuLK. 2002. 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