{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:41:40Z","timestamp":1790422900912,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,4,24]],"date-time":"2017-04-24T00:00:00Z","timestamp":1492992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n                    <jats:named-content content-type=\"genus-species\">auris<\/jats:named-content>\n                    , a new multidrug-resistant\n                    <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n                    spp. which is associated with invasive infection and high rates of mortality, has recently emerged. Here, we determined the virulence factors (germination, adherence, biofilm formation, phospholipase and proteinase production) of 16\n                    <jats:named-content content-type=\"genus-species\">C. auris<\/jats:named-content>\n                    isolates and their susceptibilities to 11 drugs belonging to different antifungal classes, including a novel orally bioavailable 1,3-\u03b2-\n                    <jats:sc>d<\/jats:sc>\n                    -glucan synthesis inhibitor (SCY-078). We also examined the effect of SCY-078 on the growth, ultrastructure, and biofilm-forming abilities of\n                    <jats:named-content content-type=\"genus-species\">C. auris<\/jats:named-content>\n                    . Our data showed that while the tested strains did not germinate, they did produce phospholipase and proteinase in a strain-dependent manner and had a significantly reduced ability to adhere and form biofilms compared to that of\n                    <jats:named-content content-type=\"genus-species\">Candida albicans<\/jats:named-content>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.01).\n                    <jats:named-content content-type=\"genus-species\">C. auris<\/jats:named-content>\n                    isolates demonstrated reduced susceptibility to fluconazole and amphotericin B, while, in general, they were susceptible to the remaining drugs tested. SCY-078 had an MIC\n                    <jats:sub>90<\/jats:sub>\n                    of 1 mg\/liter against\n                    <jats:named-content content-type=\"genus-species\">C. auris<\/jats:named-content>\n                    and caused complete inhibition of the growth of\n                    <jats:named-content content-type=\"genus-species\">C. auris<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>\n                    . Scanning electron microscopy analysis showed that SCY-078 interrupted\n                    <jats:named-content content-type=\"genus-species\">C. auris<\/jats:named-content>\n                    cell division, with the organism forming abnormal fused fungal cells. Additionally, SCY-078 possessed potent antibiofilm activity, wherein treated biofilms demonstrated significantly reduced metabolic activity and a significantly reduced thickness compared to the untreated control (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05 for both comparisons). Our study shows that\n                    <jats:named-content content-type=\"genus-species\">C. auris<\/jats:named-content>\n                    expresses several virulence determinants (albeit to a lesser extent than\n                    <jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>\n                    ) and is resistant to fluconazole and amphotericin B. SCY-078, the new orally bioavailable antifungal, had potent antifungal\/antibiofilm activity against\n                    <jats:named-content content-type=\"genus-species\">C. auris<\/jats:named-content>\n                    , indicating that further evaluation of this antifungal is warranted.\n                  <\/jats:p>","DOI":"10.1128\/aac.02396-16","type":"journal-article","created":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T20:30:22Z","timestamp":1487709022000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":353,"title":["The Emerging Pathogen Candida auris: Growth Phenotype, Virulence Factors, Activity of Antifungals, and Effect of SCY-078, a Novel Glucan Synthesis Inhibitor, on Growth Morphology and Biofilm Formation"],"prefix":"10.1128","volume":"61","author":[{"given":"Emily","family":"Larkin","sequence":"first","affiliation":[{"name":"Center for Medical Mycology, Case Western Reserve University, and University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Hager","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, Case Western Reserve University, and University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyotsna","family":"Chandra","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, Case Western Reserve University, and University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranab K.","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, Case Western Reserve University, and University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio","family":"Retuerto","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, Case Western Reserve University, and University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iman","family":"Salem","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, Case Western Reserve University, and University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Long","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, Case Western Reserve University, and University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Isham","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, Case Western Reserve University, and University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4493-4652","authenticated-orcid":false,"given":"Laura","family":"Kovanda","sequence":"additional","affiliation":[{"name":"Astellas Pharma Global Development, Inc., Northbrook, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katyna","family":"Borroto-Esoda","sequence":"additional","affiliation":[{"name":"Scynexis Inc., Jersey City, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Wring","sequence":"additional","affiliation":[{"name":"Scynexis Inc., Jersey City, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Angulo","sequence":"additional","affiliation":[{"name":"Scynexis Inc., Jersey City, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Ghannoum","sequence":"additional","affiliation":[{"name":"Center for Medical Mycology, Case Western Reserve University, and University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Global emergence of invasive infections caused by the multidrug-resistant yeast Candida auris","author":"Centers for Disease Control and Prevention","year":"2016","unstructured":"Centers for Disease Control and Prevention. 24June2016, posting date. 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