{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:09:59Z","timestamp":1790741399858,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Overexpression of the resistance-nodulation-cell division-type efflux pump AdeABC is often associated with multidrug resistance in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Acinetobacter baumannii<\/jats:named-content>\n            and has been linked to mutations in the genes encoding the AdeRS two-component system. In a previous study, we reported that the Asp20\u2192Asn amino acid substitution in the response regulator AdeR is associated with\n            <jats:italic>adeB<\/jats:italic>\n            overexpression and reduced susceptibility to the antimicrobials levofloxacin, tigecycline, and trimethoprim-sulfamethoxazole. To further characterize the effect of the Asp20\u2192Asn substitution on antimicrobial susceptibility, the expression of the efflux genes\n            <jats:italic>adeB<\/jats:italic>\n            ,\n            <jats:italic>adeJ<\/jats:italic>\n            , and\n            <jats:italic>adeG<\/jats:italic>\n            , and substrate accumulation, four plasmid constructs [containing\n            <jats:italic>adeR<\/jats:italic>\n            (\n            <jats:italic>Asp20<\/jats:italic>\n            )\n            <jats:italic>S<\/jats:italic>\n            ,\n            <jats:italic>adeR<\/jats:italic>\n            (\n            <jats:italic>Asn20<\/jats:italic>\n            )\n            <jats:italic>S<\/jats:italic>\n            ,\n            <jats:italic>adeR<\/jats:italic>\n            (\n            <jats:italic>Asp20<\/jats:italic>\n            )\n            <jats:italic>SABC<\/jats:italic>\n            , and\n            <jats:italic>adeR<\/jats:italic>\n            (\n            <jats:italic>Asn20<\/jats:italic>\n            )\n            <jats:italic>SABC<\/jats:italic>\n            ] were introduced into the\n            <jats:italic>adeRSABC<\/jats:italic>\n            -deficient\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">A. baumannii<\/jats:named-content>\n            isolate NIPH 60. Neither\n            <jats:italic>adeRS<\/jats:italic>\n            construct induced changes in antimicrobial susceptibility or substrate accumulation from that for the vector-only control. The\n            <jats:italic>adeR<\/jats:italic>\n            (\n            <jats:italic>Asp20<\/jats:italic>\n            )\n            <jats:italic>SABC<\/jats:italic>\n            transformant showed reduced susceptibility to 6 antimicrobials and accumulated 12% less ethidium than the control, whereas the Asn20 variant showed reduced susceptibility to 6 of 8 antimicrobial classes tested, and its ethidium accumulation was only 72% of that observed for the vector-only construct.\n            <jats:italic>adeB<\/jats:italic>\n            expression was 7-fold higher in the\n            <jats:italic>adeR<\/jats:italic>\n            (\n            <jats:italic>Asn20<\/jats:italic>\n            )\n            <jats:italic>SABC<\/jats:italic>\n            transformant than in its Asp20 variant. No changes in\n            <jats:italic>adeG<\/jats:italic>\n            or\n            <jats:italic>adeJ<\/jats:italic>\n            expression or in acriflavine or rhodamine 6G accumulation were detected. The antimicrobial susceptibility data suggest that AdeRS does not regulate any resistance determinants other than AdeABC. Furthermore, the characterization of the Asp20\u2192Asn20 substitution proves that the reduced antimicrobial susceptibility previously associated with this substitution was indeed caused by enhanced efflux activity of AdeB.\n          <\/jats:p>","DOI":"10.1128\/aac.02413-15","type":"journal-article","created":{"date-parts":[[2015,12,8]],"date-time":"2015-12-08T15:05:43Z","timestamp":1449587143000},"page":"1085-1090","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["The Asp20-to-Asn Substitution in the Response Regulator AdeR Leads to Enhanced Efflux Activity of AdeB in Acinetobacter baumannii"],"prefix":"10.1128","volume":"60","author":[{"given":"Jennifer","family":"Nowak","sequence":"first","affiliation":[{"name":"Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thamarai","family":"Schneiders","sequence":"additional","affiliation":[{"name":"Division of Infection and Pathway Medicine, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Seifert","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany"},{"name":"German Centre For Infection Research, Bonn-Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul G.","family":"Higgins","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00058-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/502205"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra070741"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01388-10"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1789"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-200"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/595977"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku170"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(98)80083-0"},{"key":"e_1_3_2_11_2","first-page":"107","article-title":"The RND permease superfamily: an ancient, ubiquitous and diverse family that includes human disease and development proteins","volume":"1","author":"Tseng TT","year":"1999","unstructured":"Tseng TT, Gratwick KS, Kollman J, Park D, Nies DH, Goffeau A, Saier MH, Jr. 1999. 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