{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:05:06Z","timestamp":1792670706352,"version":"4.5.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hepatitis E virus (HEV) is the causative agent of hepatitis E in humans and a member of the genus\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Orthohepevirus<\/jats:named-content>\n                    in the family\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Hepeviridae<\/jats:named-content>\n                    . HEV infections are the common cause of acute hepatitis but can also take chronic courses. Ribavirin is the treatment of choice for most patients, and type I interferon (IFN) has been evaluated in a few infected transplant patients\n                    <jats:italic>in vivo<\/jats:italic>\n                    . In this study, the antiviral effects of different exogenously administered interferons were investigated by using state-of-the-art subgenomic replicon and full-length HEV genome cell culture models. Hepatitis C virus (HCV) subgenomic replicons based on the genotype 2a JFH1 isolate served as the reference. The experiments revealed that HEV RNA replication was inhibited by the application of all types of IFN, including IFN-\u03b1 (type I), IFN-\u03b3 (type II), and IFN-\u03bb3 (type III), but to a far lesser extent than HCV replication. Simultaneous determination of interferon-stimulated gene (ISG) expression levels for all IFN types demonstrated efficient downregulation by HEV. Furthermore, different IFN-\u03b1 subtypes were also able to block viral replication in combination with ribavirin. The IFN-\u03b1 subtypes 2a and 2b exerted the strongest antiviral activity against HEV. In conclusion, these data demonstrate for the first time moderate anti-HEV activities of types II and III IFNs and different IFN-\u03b1 subtypes. As HEV employed a potent anti-interferon mechanism by restricting ISG expression, exogenous application of IFNs as immunotherapy should be carefully assessed.\n                  <\/jats:p>","DOI":"10.1128\/aac.02427-15","type":"journal-article","created":{"date-parts":[[2016,1,19]],"date-time":"2016-01-19T22:58:43Z","timestamp":1453244323000},"page":"2132-2139","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":97,"title":["Antiviral Activities of Different Interferon Types and Subtypes against Hepatitis E Virus Replication"],"prefix":"10.1128","volume":"60","author":[{"given":"Daniel","family":"Todt","sequence":"first","affiliation":[{"name":"Institute for Experimental Virology, Twincore Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Fran\u00e7ois","sequence":"additional","affiliation":[{"name":"EA4294, Laboratoire de Virologie, Centre Hospitalier Universitaire et Universite de Picardie Jules Verne, Amiens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Anggakusuma","sequence":"additional","affiliation":[{"name":"Institute for Experimental Virology, Twincore Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Behrendt","sequence":"additional","affiliation":[{"name":"Institute for Experimental Virology, Twincore Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI), 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