{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:00:57Z","timestamp":1791313257285,"version":"4.3.1"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T00:00:00Z","timestamp":1553644800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"OpGen, Inc."}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2019,4]]},"abstract":"\n We developed a rapid high-throughput PCR test and evaluated highly antibiotic-resistant clinical isolates of\n Escherichia coli<\/jats:named-content>\n (\n n<\/jats:italic>\n \u2009= 2,919),\n Klebsiella pneumoniae<\/jats:named-content>\n (\n n<\/jats:italic>\n \u2009= 1,974),\n Proteus mirabilis<\/jats:named-content>\n (\n n<\/jats:italic>\n \u2009= 1,150), and\n Pseudomonas aeruginosa<\/jats:named-content>\n (\n n<\/jats:italic>\n \u2009= 1,484) for several antibiotic resistance genes for comparison with phenotypic resistance across penicillins, cephalosporins, carbapenems, aminoglycosides, trimethoprim-sulfamethoxazole, fluoroquinolones, and macrolides. The isolates originated from hospitals in North America (34%), Europe (23%), Asia (13%), South America (12%), Africa (7%), or Oceania (1%) or were of unknown origin (9%).\n <\/jats:p>","DOI":"10.1128\/aac.02462-18","type":"journal-article","created":{"date-parts":[[2019,1,25]],"date-time":"2019-01-25T12:52:20Z","timestamp":1548420740000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Predicting Antibiotic Resistance in Gram-Negative Bacilli from Resistance Genes"],"prefix":"10.1128","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0598-5876","authenticated-orcid":false,"given":"G. Terrance","family":"Walker","sequence":"first","affiliation":[{"name":"OpGen, Inc., Gaithersburg, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Quan","sequence":"additional","affiliation":[{"name":"OpGen, Inc., Gaithersburg, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen G.","family":"Higgins","sequence":"additional","affiliation":[{"name":"OpGen, Inc., Gaithersburg, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil","family":"Toraskar","sequence":"additional","affiliation":[{"name":"OpGen, Inc., Gaithersburg, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weizhong","family":"Chang","sequence":"additional","affiliation":[{"name":"OpGen, Inc., Gaithersburg, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Saeed","sequence":"additional","affiliation":[{"name":"OpGen, Inc., Gaithersburg, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim","family":"Sapiro","sequence":"additional","affiliation":[{"name":"OpGen, Inc., Gaithersburg, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsey","family":"Pitzer","sequence":"additional","affiliation":[{"name":"OpGen, Inc., Gaithersburg, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Whitfield","sequence":"additional","affiliation":[{"name":"OpGen, Inc., Gaithersburg, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bert K.","family":"Lopansri","sequence":"additional","affiliation":[{"name":"Intermountain Medical Center, Murray, Utah, USA"},{"name":"University of Utah, Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Motyl","sequence":"additional","affiliation":[{"name":"Merck & Co., Inc., Whitehouse Station, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Sahm","sequence":"additional","affiliation":[{"name":"IHMA, Inc., Schaumburg, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","unstructured":"Centers for Disease Control and Prevention. 2013. Antibiotic resistance threats in the United States 2013. Centers for Disease Control and Prevention Atlanta GA. https:\/\/www.cdc.gov\/drugresistance\/threat-report-2013\/pdf\/ar-threats-2013-508.pdf."},{"key":"e_1_3_1_3_2","unstructured":"Centers for Disease Control and Prevention. 2015. Facility guidance for control of carbapenem-resistant Enterobacteriaceae (CRE): November 2015 update - CRE toolkit. Centers for Disease Control and Prevention Atlanta GA. https:\/\/www.cdc.gov\/hai\/pdfs\/cre\/cre-guidance-508.pdf."},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01329-17"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1708558114"},{"key":"e_1_3_1_6_2","volume-title":"Performance standards for antimicrobial susceptibility testing, 27th ed. CLSI supplement M100","author":"CLSI","year":"2017","unstructured":"CLSI. 2017. Performance standards for antimicrobial susceptibility testing, 27th ed. CLSI supplement M100. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn656"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00419-13"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr290"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03117-13"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10063"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep46327"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt180"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03954-14"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02462-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02462-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:38:37Z","timestamp":1645479517000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.02462-18"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1128\/AAC.02462-18"],"URL":"https:\/\/doi.org\/10.1128\/aac.02462-18","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4]]},"assertion":[{"value":"2018-11-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02462-18"}}