{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:36:22Z","timestamp":1790422582947,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100005564","name":"Gilead Sciences","doi-asserted-by":"publisher","award":["Amsterdam"],"award-info":[{"award-number":["Amsterdam"]}],"id":[{"id":"10.13039\/100005564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Using an immunocompetent murine model of invasive aspergillosis (IA), we previously reported that the efficacy of liposomal amphotericin B (L-AmB) (Ambisome) is not hampered by the presence of azole resistance mutations in\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            (S. Seyedmousavi, W. J. G. Melchers, J. W. Mouton, and P. E. Verweij, Antimicrob Agents Chemother 57:1866\u20131871, 2013,\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1128\/AAC.02226-12\">https:\/\/doi.org\/10.1128\/AAC.02226-12<\/jats:ext-link>\n            ). We here investigated the role of immune suppression, i.e., neutropenia and steroid treatment, in L-AmB efficacy in mice infected with wild-type (WT)\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            and with azole-resistant\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            harboring a TR\n            <jats:sub>34<\/jats:sub>\n            \/L98H mutation in the\n            <jats:italic>cyp-51A<\/jats:italic>\n            gene. Survival of treated animals at day 14 in both immunosuppressed models was significantly better than that of nontreated controls. A dose-response relationship was observed that was independent of the azole-resistant mechanism and the immunosuppression method used. In the neutropenic model, 100% survival was reached at an L-AmB dose of 16 mg\/kg of body weight for the WT strain and the TR\n            <jats:sub>34<\/jats:sub>\n            \/L98H isolate. In the steroid-treated group, 90.9% survival and 100% survival were achieved for the WT isolate and the TR\n            <jats:sub>34<\/jats:sub>\n            \/L98H isolate with an L-AmB dose of 16 mg\/kg, respectively. The 50% effective dose (ED\n            <jats:sub>50<\/jats:sub>\n            ) was 1.40 mg\/kg (95% confidence interval [CI], 0.66 to 3.00 mg\/kg) for the WT isolate and 1.92 mg\/kg (95% CI, 0.60 to 6.17 mg\/kg) for the TR\n            <jats:sub>34<\/jats:sub>\n            \/L98H isolate in the neutropenic model and was 2.40 mg\/kg (95% CI, 1.93 to 2.97 mg\/kg) for the WT isolate and 2.56 mg\/kg (95% CI, 1.43 to 4.56 mg\/kg) for the TR\n            <jats:sub>34<\/jats:sub>\n            \/L98H isolate in the steroid-treated group. Overall, there were no significant differences between the two different immunosuppressed conditions in the efficacy of L-AmB against the wild-type and azole-resistant isolates (\n            <jats:italic>P<\/jats:italic>\n            &gt; 0.9). However, the required L-AmB exposure was significantly higher than that seen in the immunocompetent model.\n          <\/jats:p>","DOI":"10.1128\/aac.02479-16","type":"journal-article","created":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T00:55:21Z","timestamp":1492476921000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["<i>In Vivo<\/i>\n            Efficacy of Liposomal Amphotericin B against Wild-Type and Azole-Resistant Aspergillus fumigatus Isolates in Two Different Immunosuppression Models of Invasive Aspergillosis"],"prefix":"10.1128","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6194-7447","authenticated-orcid":false,"given":"Seyedmojtaba","family":"Seyedmousavi","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, the Netherlands"},{"name":"Centre of Expertise in Mycology, Radboudumc\/CWZ, Nijmegen, the Netherlands"},{"name":"Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, the Netherlands"},{"name":"Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan W.","family":"Mouton","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Medical Centre, Nijmegen, the Netherlands"},{"name":"Centre of Expertise in Mycology, Radboudumc\/CWZ, Nijmegen, the Netherlands"},{"name":"Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem J. 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