{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:46:51Z","timestamp":1777441611061,"version":"3.51.4"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01-AI088025"],"award-info":[{"award-number":["R01-AI088025"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The exogenously acquired 16S rRNA methyltransferases RmtD, RmtD2, and RmtG were cloned and heterologously expressed in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n            , and the recombinant proteins were purified to near homogeneity. Each methyltransferase conferred an aminoglycoside resistance profile consistent with m\n            <jats:sup>7<\/jats:sup>\n            G1405 modification, and this activity was confirmed by\n            <jats:italic>in<\/jats:italic>\n            <jats:italic>vitro<\/jats:italic>\n            30S methylation assays. Analyses of protein structure and interaction with\n            <jats:italic>S<\/jats:italic>\n            -adenosyl-\n            <jats:sc>l<\/jats:sc>\n            -methionine suggest that the molecular mechanisms of substrate recognition and catalysis are conserved across the 16S rRNA (m\n            <jats:sup>7<\/jats:sup>\n            G1405) methyltransferase family.\n          <\/jats:p>","DOI":"10.1128\/aac.02482-15","type":"journal-article","created":{"date-parts":[[2015,11,10]],"date-time":"2015-11-10T04:09:28Z","timestamp":1447128568000},"page":"699-702","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Heterologous Expression and Functional Characterization of the Exogenously Acquired Aminoglycoside Resistance Methyltransferases RmtD, RmtD2, and RmtG"],"prefix":"10.1128","volume":"60","author":[{"given":"La\u00eds L.","family":"Corr\u00eaa","sequence":"first","affiliation":[{"name":"Department of 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