{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:32:45Z","timestamp":1790616765693,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Rambam Health Care Center","award":["Ofakim"],"award-info":[{"award-number":["Ofakim"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Treatment of\n            <jats:named-content content-type=\"genus-species\">Mycobacterium abscessus<\/jats:named-content>\n            infections is extremely challenging due to its intrinsic resistance to most antibiotics, and research of pathogenesis is limited due to a lack of a practical\n            <jats:italic>in vivo<\/jats:italic>\n            model of infection. The objective of this study was to establish a simple\n            <jats:italic>in vivo<\/jats:italic>\n            model for\n            <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n            infection, virulence, and drug testing in\n            <jats:named-content content-type=\"genus-species\">Galleria mellonella<\/jats:named-content>\n            larvae. We inoculated larvae with\n            <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n            bacteria and assessed histopathology, CFU count, and mortality with and without antibiotic treatment. We also constructed a luminescent, recombinant\n            <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n            mutant, mDB158, and imaged infected larvae using the IVIS\n            <jats:italic>in vivo<\/jats:italic>\n            imaging system.\n            <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n            proliferated and induced granuloma-like responses in infected larvae, leading to larval mortality. The\n            <jats:named-content content-type=\"genus-species\">G. mellonella<\/jats:named-content>\n            model was further validated successfully by demonstration of the expected favorable antimicrobial effect of treatment with meropenem and the superiority of combination treatment (meropenem and tigecycline) over that with single agents. We then used IVIS imaging of larvae infected with luminescent\n            <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n            , allowing live real-time assessment of bacterial load. We used this method to compare the antimicrobial effects of various antibiotics (meropenem, amikacin, linezolid, levofloxacin, etc.) on bacterial proliferation and larval survival. Meropenem and amikacin had the most favorable effects, correlating well with common clinical practice guidelines. These findings suggest\n            <jats:named-content content-type=\"genus-species\">G. mellonella<\/jats:named-content>\n            to be an excellent\n            <jats:italic>in vivo<\/jats:italic>\n            model for research of\n            <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n            infection, pathogenesis, and treatment. Luminescent\n            <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n            and IVIS imaging further facilitates this model. Results obtained in this model clearly substantiated common clinical practice, thus validating the model as a predictor of treatment efficacy and outcome.\n          <\/jats:p>","DOI":"10.1128\/aac.02539-17","type":"journal-article","created":{"date-parts":[[2018,2,6]],"date-time":"2018-02-06T09:45:59Z","timestamp":1517910359000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Establishment and Validation of Galleria mellonella as a Novel Model Organism To Study Mycobacterium abscessus Infection, Pathogenesis, and Treatment"],"prefix":"10.1128","volume":"62","author":[{"given":"Michal","family":"Meir","sequence":"first","affiliation":[{"name":"The Ruth Rappaport Children's Hospital, Rambam Health Care Campus, Haifa, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana","family":"Grosfeld","sequence":"additional","affiliation":[{"name":"Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Barkan","sequence":"additional","affiliation":[{"name":"Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200604-571ST"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2015-207360"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2009.12.001"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.12140"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00100"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2013.06.011"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku510"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/02713680500477321"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2016.1203486"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01353-17"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029774"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01521-05"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00400-10"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01260-10"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002598"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010777"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit614"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00014-07"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1007696"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2015.1135289"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112179"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.3791\/4392"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2015.1025192"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2015.1081330"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b13-00270"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr198"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku482"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv111"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.23629"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02790-13"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02539-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02539-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:11:32Z","timestamp":1645477892000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.02539-17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1128\/AAC.02539-17"],"URL":"https:\/\/doi.org\/10.1128\/aac.02539-17","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4]]},"assertion":[{"value":"2017-12-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02539-17"}}