{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T20:00:29Z","timestamp":1760385629719,"version":"3.41.2"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            New Delhi metallo-\u03b2-lactamase 1 (NDM-1) is a subclass B1 metallo-\u03b2-lactamase that exhibits a broad spectrum of activity against \u03b2-lactam antibiotics. Here we report the kinetic study of 6 Q119X variants obtained by site-directed mutagenesis of NDM-1. All Q119X variants were able to hydrolyze carbapenems, penicillins and first-, second-, third-, and fourth-generation cephalosporins very efficiently. In particular, Q119E, Q119Y, Q119V, and Q119K mutants showed improvements in\n            <jats:italic>k<\/jats:italic>\n            <jats:sub>\n              cat\n              <jats:italic>\/<\/jats:italic>\n            <\/jats:sub>\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            values for penicillins, compared with NDM-1. The catalytic efficiencies of the Q119K variant for benzylpenicillin and carbenicillin were about 65- and 70-fold higher, respectively, than those of NDM-1. The Q119K and Q119Y enzymes had\n            <jats:italic>k<\/jats:italic>\n            <jats:sub>cat<\/jats:sub>\n            \/\n            <jats:italic>\n              K\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            values for ceftazidime about 25- and 89-fold higher, respectively, than that of NDM-1.\n          <\/jats:p>","DOI":"10.1128\/aac.02541-17","type":"journal-article","created":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T09:35:37Z","timestamp":1526636137000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["A Kinetic Study of the Replacement by Site Saturation Mutagenesis of Residue 119 in NDM-1 Metallo-\u03b2-Lactamase"],"prefix":"10.1128","volume":"62","author":[{"given":"Francesca","family":"Marcoccia","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Universit\u00e0 degli Studi dell'Aquila, L'Aquila, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola Sandra","family":"Mercuri","sequence":"additional","affiliation":[{"name":"Laboratoire de Macromol\u00e9cules Biologiques, Centre d'Ing\u00e9nierie des Prot\u00e9ines, Universit\u00e9 de Li\u00e8ge, Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moreno","family":"Galleni","sequence":"additional","affiliation":[{"name":"Laboratoire de Macromol\u00e9cules Biologiques, Centre d'Ing\u00e9nierie des Prot\u00e9ines, Universit\u00e9 de Li\u00e8ge, Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Celenza","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Universit\u00e0 degli Studi dell'Aquila, L'Aquila, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianfranco","family":"Amicosante","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Universit\u00e0 degli Studi dell'Aquila, L'Aquila, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariagrazia","family":"Perilli","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Universit\u00e0 degli Studi dell'Aquila, L'Aquila, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"doi-asserted-by":"publisher","key":"e_1_3_2_2_2","DOI":"10.4155\/fmc-2016-0015"},{"doi-asserted-by":"publisher","key":"e_1_3_2_3_2","DOI":"10.1016\/j.ijantimicag.2016.03.009"},{"doi-asserted-by":"publisher","key":"e_1_3_2_4_2","DOI":"10.1038\/emi.2017.43"},{"doi-asserted-by":"publisher","key":"e_1_3_2_5_2","DOI":"10.1186\/s12866-017-1012-8"},{"key":"e_1_3_2_6_2","first-page":"S30","article-title":"Recent epidemic emergence of bla\n                  NDM-1 metallo-\u03b2-lactamase in enteric organisms from India is mostly linked to A\/C plasmids","volume":"16","author":"Toleman M","year":"2010","unstructured":"Toleman M, Karthikeyan K, Sharma M, Chaudhary U, Thirunarayan M, Krishnan P, Walsh T. 2010. Recent epidemic emergence of blaNDM-1 metallo-\u03b2-lactamase in enteric organisms from India is mostly linked to A\/C plasmids. Clin Microbiol Infect 16(Suppl 2):S30.","journal-title":"Clin Microbiol Infect"},{"doi-asserted-by":"publisher","key":"e_1_3_2_7_2","DOI":"10.1016\/j.mib.2010.09.006"},{"doi-asserted-by":"publisher","key":"e_1_3_2_8_2","DOI":"10.1128\/AAC.00774-09"},{"doi-asserted-by":"publisher","key":"e_1_3_2_9_2","DOI":"10.1002\/pro.697"},{"doi-asserted-by":"publisher","key":"e_1_3_2_10_2","DOI":"10.1096\/fj.11-184036"},{"doi-asserted-by":"publisher","key":"e_1_3_2_11_2","DOI":"10.1096\/fj.12-224014"},{"doi-asserted-by":"publisher","key":"e_1_3_2_12_2","DOI":"10.1007\/s13238-011-1055-9"},{"doi-asserted-by":"publisher","key":"e_1_3_2_13_2","DOI":"10.1128\/AAC.48.7.2347-2349.2004"},{"doi-asserted-by":"publisher","key":"e_1_3_2_14_2","DOI":"10.1039\/c3cp55069a"},{"doi-asserted-by":"publisher","key":"e_1_3_2_15_2","DOI":"10.1016\/0378-1119(89)90358-2"},{"doi-asserted-by":"publisher","key":"e_1_3_2_16_2","DOI":"10.1128\/AAC.00531-15"},{"key":"e_1_3_2_17_2","volume-title":"Document M07-A9","author":"Clinical and Laboratory Standards Institute","year":"2012","unstructured":"Clinical and Laboratory Standards Institute. 2012. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard\u2014 9th ed. Document M07-A9. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"9"},{"key":"e_1_3_2_18_2","volume-title":"Biochemical calculations: how to solve mathematical problems in general biochemistry","author":"Segel IH","year":"1976","unstructured":"Segel IH. 1976. Enzyme kinetics. Biochemical calculations: how to solve mathematical problems in general biochemistry, 2nd ed, p 236\u2013241. John Wiley & Sons, New York, NY.","edition":"2"},{"doi-asserted-by":"publisher","key":"e_1_3_2_19_2","DOI":"10.1128\/AAC.01977-13"},{"doi-asserted-by":"publisher","key":"e_1_3_2_20_2","DOI":"10.1016\/0006-2952(87)90609-5"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02541-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02541-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:23:57Z","timestamp":1645478637000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.02541-17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":19,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1128\/AAC.02541-17"],"URL":"https:\/\/doi.org\/10.1128\/aac.02541-17","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"type":"print","value":"0066-4804"},{"type":"electronic","value":"1098-6596"}],"subject":[],"published":{"date-parts":[[2018,8]]},"assertion":[{"value":"2017-12-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02541-17"}}