{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:32:07Z","timestamp":1759336327522,"version":"3.41.2"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100004441","name":"The Swedish International Development Cooperation Agency","doi-asserted-by":"crossref","award":["01014"],"award-info":[{"award-number":["01014"]}],"id":[{"id":"10.13039\/100004441","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001729","name":"Swedish Foundation for Strategic Research","doi-asserted-by":"crossref","award":["RBd08-0014"],"award-info":[{"award-number":["RBd08-0014"]}],"id":[{"id":"10.13039\/501100001729","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001862","name":"The Swedish Research Council","doi-asserted-by":"crossref","award":["K2014-67X-11217-20-3"],"award-info":[{"award-number":["K2014-67X-11217-20-3"]}],"id":[{"id":"10.13039\/501100001862","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100009054","name":"icddr,b","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009054","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004047","name":"Karolinska Institutet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have shown previously that oral treatment with sodium butyrate or phenylbutyrate in an experimental model of shigellosis improves clinical outcomes and induces the expression of the antimicrobial peptide CAP-18 in the large intestinal epithelia. In a subsequent study, we found that entinostat, an aroylated phenylenediamine compound, has similar therapeutic potential against shigellosis. In this study, we aimed to evaluate entinostat as a potential candidate for host-directed therapy against cholera in an experimental model.\n            <jats:named-content content-type=\"genus-species\">Vibrio cholerae<\/jats:named-content>\n            -infected rabbits were treated with two different dose regimens of entinostat: either 0.5 mg twice daily for 2 days or 1 mg once daily for 2 days. The effects of treatment on clinical outcomes and\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            shedding (CFU count in stool) were observed. Immunohistochemical analysis was carried out to assess CAP-18 expression in ileal and jejunal mucosae. The serum zonulin level was measured by an enzyme-linked immunosorbent assay (ELISA) to evaluate gut permeability. Infection of rabbits with\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            downregulated CAP-18 expression in the ileal epithelium; the expression was replenished by oral treatment with entinostat at either dose regimen. The level of zonulin, a marker of gut permeability, in serum was upregulated after infection, and this upregulation was counteracted after treatment with entinostat. Entinostat treatment also led to recovery from cholera and a decline in the\n            <jats:named-content content-type=\"genus-species\">V. cholerae<\/jats:named-content>\n            count in stool. In conclusion, the improved clinical outcome of cholera for rabbits treated with entinostat is associated with the induction of CAP-18 and the reduction of gut epithelial permeability.\n          <\/jats:p>","DOI":"10.1128\/aac.02570-16","type":"journal-article","created":{"date-parts":[[2017,4,25]],"date-time":"2017-04-25T00:55:26Z","timestamp":1493081726000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Treatment with Entinostat Heals Experimental Cholera by Affecting Physical and Chemical Barrier Functions of Intestinal Epithelia"],"prefix":"10.1128","volume":"61","author":[{"given":"Protim","family":"Sarker","sequence":"first","affiliation":[{"name":"Infectious Diseases Division, icddr,b, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atanu","family":"Banik","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, icddr,b, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Stromberg","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gudmundur H.","family":"Gudmundsson","sequence":"additional","affiliation":[{"name":"Institute of Biology and Biomedical Center, University of Iceland, Reykjavik, Iceland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rubhana","family":"Raqib","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, icddr,b, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgitta","family":"Agerberth","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Division of Clinical Microbiology (F68), Karolinska Institutet, Karolinska University Hospital Huddinge, Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"433","article-title":"Cholera, 2015","volume":"91","author":"World Health Organization","year":"2016","unstructured":"World Health Organization. 2016. Cholera, 2015. Wkly Epidemiol Rec91:433\u2013440.","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI106874"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.60.1.167-215.1996"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.12.5242"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1996.0047"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15328-7"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp011603"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.1.95-110.2002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/415389a"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/84627"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071698"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2013.08.007"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01227.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.06.014"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602888103"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020637"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-111"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/1753425913493338"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep36692"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.32.2.739-747.1981"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.8.2727-2736.1991"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00003.2008"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2012.08.012"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10517-015-3045-z"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2003.12069.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2005.11.014"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2004.05.005"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00818-09"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep33274"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2008.10.028"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-158972"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2000.00025.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02169-3"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.2527\/jas.50965"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2004.12.004"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2010.195"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2015.00057"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.03.041"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-05-021873"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-1461"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2011.527"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.38.9411"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.43.7251"},{"key":"e_1_3_2_45_2","first-page":"9360","article-title":"The effect of MS-275 on CYP450 isoforms activity in rats by cocktail method","volume":"8","author":"Wu Q","year":"2015","unstructured":"Wu Q ZhangQWenCHuLWangXLinG. 2015. The effect of MS-275 on CYP450 isoforms activity in rats by cocktail method. Int J Clin Exp Pathol8:9360\u20139367.","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_3_2_46_2","volume-title":"Guide for the care and use of laboratory animals","author":"National Research Council","year":"2011","unstructured":"National Research Council. 2011. Guide for the care and use of laboratory animals,8th ed. National Academies Press,Washington, DC.","edition":"8"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02570-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02570-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:02:50Z","timestamp":1645477370000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.02570-16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":45,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1128\/AAC.02570-16"],"URL":"https:\/\/doi.org\/10.1128\/aac.02570-16","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"type":"print","value":"0066-4804"},{"type":"electronic","value":"1098-6596"}],"subject":[],"published":{"date-parts":[[2017,7]]},"assertion":[{"value":"2016-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-04-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-06-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02570-16"}}