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The U.S. CDC now regards developing candidal antifungal resistance as a threat, creating a need for new and more effective antifungal treatments. Iron is an essential nutrient for all living cells, and there is growing evidence that interference with iron homeostasis of<jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>can improve its response to antifungals. This study was aimed at establishing whether withholding iron by currently used medical iron chelators and the novel chelator DIBI could restrict growth and also enhance the activity of azoles against clinical isolates of<jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>. DIBI, but not deferoxamine or deferiprone, inhibited the growth of<jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>at relatively low concentrations<jats:italic>in vitro<\/jats:italic>, and this inhibition was reversed by iron addition. DIBI in combination with various azoles demonstrated stronger growth inhibition than the azoles alone and greatly prolonged the inhibition of cell multiplication. In addition, the administration of DIBI along with fluconazole (FLC) to mice inoculated with an FLC-sensitive isolate in a model of experimental<jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>vaginitis showed a markedly improved clearance of infection. These results suggest that iron chelation by DIBI has the potential to enhance azole efficacy for the treatment of candidiasis.<\/jats:p>","DOI":"10.1128\/aac.02576-17","type":"journal-article","created":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T10:12:31Z","timestamp":1527502351000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Iron Restriction to Clinical Isolates of Candida albicans by the Novel Chelator DIBI Inhibits Growth and Increases Sensitivity to Azoles<i>In Vitro<\/i>and<i>In Vivo<\/i>in a Murine Model of Experimental Vaginitis"],"prefix":"10.1128","volume":"62","author":[{"given":"Kimberley A.","family":"Savage","sequence":"first","affiliation":[{"name":"Chelation Partners Inc., Halifax, Nova Scotia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria del Carmen","family":"Parquet","sequence":"additional","affiliation":[{"name":"Chelation Partners Inc., Halifax, Nova Scotia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Allan","sequence":"additional","affiliation":[{"name":"Chelation Partners Inc., Halifax, Nova Scotia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross J.","family":"Davidson","sequence":"additional","affiliation":[{"name":"Nova Scotia Health Authority, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada"},{"name":"Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce E.","family":"Holbein","sequence":"additional","affiliation":[{"name":"Chelation Partners Inc., Halifax, Nova Scotia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Lilly","sequence":"additional","affiliation":[{"name":"Louisiana State University Health Sciences Center School of Dentistry, New Orleans, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Paul L.","family":"Fidel","sequence":"additional","affiliation":[{"name":"Louisiana State University Health Sciences Center School of Dentistry, New Orleans, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00029-06"},{"key":"e_1_3_3_3_2","volume-title":"Antibiotic resistance threats in the United States","author":"Centers for Disease Control and Prevention","year":"2013","unstructured":"Centers for Disease Control and Prevention. 2013. 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