{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:33:59Z","timestamp":1790674439808,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Carbapenem-resistant\n            <jats:named-content content-type=\"genus-species\">Enterobacter cloacae<\/jats:named-content>\n            complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by PCR for seven common carbapenemase gene groups, namely, KPC, NDM, OXA-48, VIM, IMP, GES, and NMC-A\/IMI. Nineteen of the submitted isolates (1.7%) were found to harbor Ambler class A\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NMC-A<\/jats:sub>\n            or\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMI<\/jats:sub>\n            -type carbapenemases. All 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprofloxacin. Most isolates (17\/19) gave positive results with the Carba-NP test for phenotypic carbapenemase detection. Isolates were genetically diverse by pulsed-field gel electrophoresis macrorestriction analysis, multilocus sequence typing, and\n            <jats:italic>hsp60<\/jats:italic>\n            gene analysis. The genes were found in various\n            <jats:named-content content-type=\"genus-species\">Enterobacter cloacae<\/jats:named-content>\n            complex species; however,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NMC-A<\/jats:sub>\n            was highly associated with\n            <jats:named-content content-type=\"genus-species\">Enterobacter ludwigii<\/jats:named-content>\n            . Whole-genome sequencing and bioinformatics analysis revealed that all NMC-A (\n            <jats:italic>n<\/jats:italic>\n            = 10), IMI-1 (\n            <jats:italic>n<\/jats:italic>\n            = 5), and IMI-9 (\n            <jats:italic>n<\/jats:italic>\n            = 2) producers harbored the carbapenemase gene on EludIMEX-1-like integrative mobile elements (EcloIMEXs) located in the identical chromosomal locus. Two novel genes,\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMI-5<\/jats:sub>\n            and\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>IMI-6<\/jats:sub>\n            , were harbored on different IncFII-type plasmids.\n            <jats:named-content content-type=\"genus-species\">Enterobacter cloacae<\/jats:named-content>\n            complex isolates harboring\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>NMC-A\/IMI<\/jats:sub>\n            -type carbapenemases are relatively rare in Canada. Though mostly found integrated into the chromosome, some variants are located on plasmids that may enhance their mobility potential.\n          <\/jats:p>","DOI":"10.1128\/aac.02578-16","type":"journal-article","created":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T01:30:22Z","timestamp":1487727022000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Enterobacter cloacae Complex Isolates Harboring\n            <i>bla<\/i>\n            <sub>NMC-A<\/sub>\n            or\n            <i>bla<\/i>\n            <sub>IMI<\/sub>\n            -Type Class A Carbapenemase Genes on Novel Chromosomal Integrative Elements and Plasmids"],"prefix":"10.1128","volume":"61","author":[{"given":"David A.","family":"Boyd","sequence":"first","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura F.","family":"Mataseje","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross","family":"Davidson","sequence":"additional","affiliation":[{"name":"Queen Elizabeth II Health Sciences Centre, Dalhousie University, Halifax, Nova Scotia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes A.","family":"Delport","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Pathology and Laboratory Medicine, London Health Sciences Centre, London, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff","family":"Fuller","sequence":"additional","affiliation":[{"name":"Provincial Laboratory of Alberta, Alberta Health Services, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Hoang","sequence":"additional","affiliation":[{"name":"British Columbia Public Health Microbiology and Reference Laboratory, Provincial Health Services Authority Laboratories, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"Laboratoire de Sant\u00e9 Publique du Qu\u00e9bec, Sainte-Anne-de-Bellevue, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul N.","family":"Levett","sequence":"additional","affiliation":[{"name":"Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane L.","family":"Roscoe","sequence":"additional","affiliation":[{"name":"Pathology and Laboratory Medicine, Vancouver General Hospital, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara M.","family":"Willey","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Mount Sinai Hospital, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Mulvey","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, 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