{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:15:11Z","timestamp":1791191711944,"version":"4.1.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Enterococcal implant-associated infections are difficult to treat because antibiotics generally lack activity against enterococcal biofilms. We investigated fosfomycin, rifampin, and their combinations against planktonic and adherent\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Enterococcus faecalis<\/jats:named-content>\n            (ATCC 19433)\n            <jats:italic>in vitro<\/jats:italic>\n            and in a foreign-body infection model. The MIC\/MBC\n            <jats:sub>log<\/jats:sub>\n            values were 32\/&gt;512 \u03bcg\/ml for fosfomycin, 4\/&gt;64 \u03bcg\/ml for rifampin, 1\/2 \u03bcg\/ml for ampicillin, 2\/&gt;256 \u03bcg\/ml for linezolid, 16\/32 \u03bcg\/ml for gentamicin, 1\/&gt;64 \u03bcg\/ml for vancomycin, and 1\/5 \u03bcg\/ml for daptomycin. In time-kill studies, fosfomycin was bactericidal at 8\u00d7 and 16\u00d7 MIC, but regrowth of resistant strains occurred after 24 h. With the exception of gentamicin, no complete inhibition of growth-related heat production was observed with other antimicrobials on early (3 h) or mature (24 h) biofilms. In the animal model, fosfomycin alone or in combination with daptomycin reduced planktonic counts by \u22484 log\n            <jats:sub>10<\/jats:sub>\n            CFU\/ml below the levels before treatment. Fosfomycin cleared planktonic bacteria from 74% of cage fluids (i.e., no growth in aspirated fluid) and eradicated biofilm bacteria from 43% of cages (i.e., no growth from removed cages). In combination with gentamicin, fosfomycin cleared 77% and cured 58% of cages; in combination with vancomycin, fosfomycin cleared 33% and cured 18% of cages; in combination with daptomycin, fosfomycin cleared 75% and cured 17% of cages. Rifampin showed no activity on planktonic or adherent\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. faecalis<\/jats:named-content>\n            , whereas in combination with daptomycin it cured 17% and with fosfomycin it cured 25% of cages. Emergence of fosfomycin resistance was not observed\n            <jats:italic>in vivo<\/jats:italic>\n            . In conclusion, fosfomycin showed activity against planktonic and adherent\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. faecalis<\/jats:named-content>\n            . Its role against enterococcal biofilms should be further investigated, especially in combination with rifampin and\/or daptomycin treatment.\n          <\/jats:p>","DOI":"10.1128\/aac.02583-12","type":"journal-article","created":{"date-parts":[[2013,10,22]],"date-time":"2013-10-22T02:41:14Z","timestamp":1382409674000},"page":"1284-1293","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["Activities of Fosfomycin and Rifampin on Planktonic and Adherent Enterococcus faecalis Strains in an Experimental Foreign-Body Infection Model"],"prefix":"10.1128","volume":"58","author":[{"given":"Alessandra","family":"Oliva","sequence":"first","affiliation":[{"name":"Service of Infectious Diseases, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland"},{"name":"Center for Musculoskeletal Surgery, Charit\u00e9\u2014University Medicine, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrika","family":"Furustrand Tafin","sequence":"additional","affiliation":[{"name":"Septic Surgical Unit, Department of Surgery and Anesthesiology, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena Maryka","family":"Maiolo","sequence":"additional","affiliation":[{"name":"Septic Surgical Unit, Department of Surgery and Anesthesiology, Lausanne University Hospital, Lausanne, Switzerland"},{"name":"Center for Musculoskeletal Surgery, Charit\u00e9\u2014University Medicine, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safaa","family":"Jeddari","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"B\u00e9trisey","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrej","family":"Trampuz","sequence":"additional","affiliation":[{"name":"Service of Infectious Diseases, Department of Medicine, Lausanne University Hospital, Lausanne, Switzerland"},{"name":"Center for Musculoskeletal Surgery, Charit\u00e9\u2014University Medicine, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05321-1"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.qco.0000235161.85925.e8"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b0133283553441"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2761"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47331-0"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03213.x"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra040181"},{"key":"e_1_3_1_10_2","first-page":"1537","article-title":"Role of rifampin for treatment of orthopedic implant-related staphylococcal infections: a randomized controlled trial","volume":"279","author":"Zimmerli W","year":"1998","unstructured":"ZimmerliWWidmerAFBlatterMFreiROchsnerPE. 1998. 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