{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:01:43Z","timestamp":1790823703427,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,8]]},"abstract":"ABSTRACT<\/jats:title>\n \n Aspergillus niger<\/jats:named-content>\n and its related species, known as\n Aspergillus<\/jats:named-content>\n section\n Nigri<\/jats:italic>\n , are ubiquitously distributed across the globe and are often isolated from clinical specimens. In Japan,\n Aspergillus<\/jats:named-content>\n section\n Nigri<\/jats:italic>\n is second most often isolated from clinical specimens following\n Aspergillus fumigatus<\/jats:named-content>\n . We determined the species of\n Aspergillus<\/jats:named-content>\n section\n Nigri<\/jats:italic>\n isolated in Japan by DNA sequencing of partial \u03b2-tubulin genes and investigated drug susceptibility by the CLSI M38-A2 method. The collection contained 20\n Aspergillus niger<\/jats:named-content>\n , 59\n Aspergillus welwitschiae<\/jats:named-content>\n , and 39\n Aspergillus tubingensis<\/jats:named-content>\n strains. Drug susceptibility testing revealed 30 to 55% of\n A. niger<\/jats:named-content>\n , 6.8 to 18.6% of\n A. welwitschiae<\/jats:named-content>\n , and 79.5 to 89.7% of\n A. tubingensis<\/jats:named-content>\n isolates to be less susceptible (so-called resistant) to itraconazole (ITC) and\/or voriconazole (VRC) according to the epidemiologic cutoff values (ECVs) proposed for\n A. niger<\/jats:named-content>\n previously. MIC distributions of ITC or VRC showed no remarkable differences between clinical and environmental isolates. When the\n cyp51A<\/jats:italic>\n sequences were compared between susceptible and resistant strains, 18 amino acid mutations were specific for resistant isolates of\n A. niger<\/jats:named-content>\n and\n A. tubingensis<\/jats:named-content>\n ; however, none of them were confirmed to be associated with azole resistance. Three nonrelated\n A. welwitschiae<\/jats:named-content>\n isolates possessed a partial deletion in\n cyp51A<\/jats:italic>\n , likely attributable to being more susceptible to azoles than other isolates. One of five ITC-resistant\n A. tubingensis<\/jats:named-content>\n isolates showed higher expression of\n cyp51A<\/jats:italic>\n than did susceptible strains. Our results show that\n cyp51A<\/jats:italic>\n point mutations may have no association with azole resistance but that in some cases the overexpression of\n cyp51A<\/jats:italic>\n may lead to the azole resistance in these species.\n <\/jats:p>","DOI":"10.1128\/aac.02583-16","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T00:30:23Z","timestamp":1497313823000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Drug Sensitivity and Resistance Mechanism in Aspergillus Section\n Nigri<\/i>\n Strains from Japan"],"prefix":"10.1128","volume":"61","author":[{"given":"Aki","family":"Hashimoto","sequence":"first","affiliation":[{"name":"Division of Clinical Research, Medical Mycology Research Center, Chiba University, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Hagiwara","sequence":"additional","affiliation":[{"name":"Division of Clinical Research, Medical Mycology Research Center, Chiba University, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Division of Clinical Research, Medical Mycology Research Center, Chiba University, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Yahiro","sequence":"additional","affiliation":[{"name":"Division of Clinical Research, Medical Mycology Research Center, Chiba University, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alimu","family":"Yikelamu","sequence":"additional","affiliation":[{"name":"Division of Bio-resources, Medical Mycology Research Center, Chiba University, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yaguchi","sequence":"additional","affiliation":[{"name":"Division of Bio-resources, Medical Mycology Research Center, Chiba University, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiko","family":"Kamei","sequence":"additional","affiliation":[{"name":"Division of Clinical Research, Medical Mycology Research Center, Chiba University, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3114\/sim.2011.69.01"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:ANTO.0000024907.85688.05"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3114\/sim.2007.59.13"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063769"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01382"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03233-14"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00304-11"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2015.12.004"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01075-16"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00156-08"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.1.214-222.2005"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.11.4405-4413.2004"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002374"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05027-11"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks451"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resinv.2015.08.007"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2010.548084"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01155-09"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00536-10"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00686-11"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myv118"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.22.5279"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.4.1323-1330.1995"},{"key":"e_1_3_2_25_2","volume-title":"Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard\u20142nd ed, CLSI document M38-A2","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard\u20142nd ed, CLSI document M38-A2. 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