{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:55:01Z","timestamp":1790801701961,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004587","name":"MINECO | Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["RD12\/0015"],"award-info":[{"award-number":["RD12\/0015"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"MINECO | Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["RD16\/0016"],"award-info":[{"award-number":["RD16\/0016"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Inhaled administration of high doses of aminoglycosides is a key maintenance treatment of\n            <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa<\/jats:named-content>\n            chronic respiratory infections in cystic fibrosis (CF). We analyzed the dynamics and mechanisms of stepwise high-level tobramycin resistance development\n            <jats:italic>in vitro<\/jats:italic>\n            and compared the results with those of isogenic pairs of susceptible and resistant clinical isolates. Resistance development correlated with\n            <jats:italic>fusA1<\/jats:italic>\n            mutations\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo. pmrB<\/jats:italic>\n            mutations, conferring polymyxin resistance, were also frequently selected\n            <jats:italic>in vitro<\/jats:italic>\n            . In contrast, mutational overexpression of MexXY, a hallmark of aminoglycoside resistance in CF, was not observed in\n            <jats:italic>in vitro<\/jats:italic>\n            evolution experiments.\n          <\/jats:p>","DOI":"10.1128\/aac.02583-17","type":"journal-article","created":{"date-parts":[[2018,1,31]],"date-time":"2018-01-31T14:03:58Z","timestamp":1517407438000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Evolution of the Pseudomonas aeruginosa Aminoglycoside Mutational Resistome\n            <i>In Vitro<\/i>\n            and in the Cystic Fibrosis Setting"],"prefix":"10.1128","volume":"62","author":[{"given":"Carla","family":"L\u00f3pez-Causap\u00e9","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Universitario Son Espases, Instituto de Investigaci\u00f3n Sanitaria de las Islas Baleares (IdISBa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Rubio","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Universitario Son Espases, Instituto de Investigaci\u00f3n Sanitaria de las Islas Baleares (IdISBa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6452-9945","authenticated-orcid":false,"given":"Gabriel","family":"Cabot","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Universitario Son Espases, Instituto de Investigaci\u00f3n Sanitaria de las Islas Baleares (IdISBa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9327-1894","authenticated-orcid":false,"given":"Antonio","family":"Oliver","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda and Unidad de Investigaci\u00f3n, Hospital Universitario Son Espases, Instituto de Investigaci\u00f3n Sanitaria de las Islas Baleares (IdISBa), Palma de Mallorca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12033"},{"key":"e_1_3_3_3_2","first-page":"433","volume-title":"Evolutionary biology of bacterial and fungal pathogens","author":"Oliver A","year":"2008","unstructured":"Oliver A, Mena A, Maci\u00e0 MD. 2008. Evolution of Pseudomonas aeruginosa pathogenicity: from acute to chronic infections, p 433\u2013444. In Baquero F, Nombela C, Cassell GH, Gut\u00ederrez JA (ed), Evolutionary biology of bacterial and fungal pathogens. 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Performance standards for antimicrobial susceptibility testing\u201427th ed, vol 37. CLSI document M100. 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