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CIP is an antibiotic of the quinolone family that is used to treat\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            infections.\n            <jats:italic>In silico<\/jats:italic>\n            analysis, performed to identify CIP resistance genes, revealed that the 65-amino-acid product encoded by the\n            <jats:italic>orf131<\/jats:italic>\n            gene in pUM505 displays 40% amino acid identity to the\n            <jats:named-content content-type=\"genus-species\">Mycobacterium smegmatis<\/jats:named-content>\n            aminoglycoside phosphotransferase (an enzyme that phosphorylates and inactivates aminoglycoside antibiotics). We cloned\n            <jats:italic>orf131<\/jats:italic>\n            (renamed\n            <jats:italic>crpP<\/jats:italic>\n            , for\n            <jats:italic>c<\/jats:italic>\n            iprofloxacin\n            <jats:italic>r<\/jats:italic>\n            esistance\n            <jats:italic>p<\/jats:italic>\n            rotein,\n            <jats:italic>p<\/jats:italic>\n            lasmid encoded) into the pUCP20 shuttle vector. The resulting recombinant plasmid, pUC-\n            <jats:italic>crpP<\/jats:italic>\n            , conferred resistance to CIP on\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            strain J53-3, suggesting that this gene encodes a protein involved in CIP resistance. Using coupled enzymatic analysis, we determined that the activity of CrpP on CIP is ATP dependent, while little activity against norfloxacin was detected, suggesting that CIP may undergo phosphorylation. Using a recombinant His-tagged CrpP protein and liquid chromatography\u2013tandem mass spectrometry, we also showed that CIP was phosphorylated prior to its degradation. Thus, our findings demonstrate that CrpP, encoded on the pUM505 plasmid, represents a new mechanism of CIP resistance in\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            , which involves phosphorylation of the antibiotic.\n          <\/jats:p>","DOI":"10.1128\/aac.02629-17","type":"journal-article","created":{"date-parts":[[2018,3,27]],"date-time":"2018-03-27T10:11:49Z","timestamp":1522145509000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid"],"prefix":"10.1128","volume":"62","author":[{"given":"V\u00edctor M.","family":"Ch\u00e1vez-Jacobo","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Morelia, Michoac\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen C.","family":"Hern\u00e1ndez-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Morelia, Michoac\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Romo-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, DCNE, Universidad de Guanajuato, Guanajuato, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roc\u00edo Viridiana","family":"P\u00e9rez-Gallardo","sequence":"additional","affiliation":[{"name":"Laboratorio de Inocuidad Qu\u00edmica, Centro de Innovaci\u00f3n y Desarrollo Agroalimentario de Michoac\u00e1n, A.C., Morelia, Michoac\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8337-5830","authenticated-orcid":false,"given":"Jes\u00fas","family":"Campos-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Morelia, Michoac\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. F\u00e9lix","family":"Guti\u00e9rrez-Corona","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, DCNE, Universidad de Guanajuato, Guanajuato, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Pablo","family":"Garc\u00eda-Merinos","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Morelia, Michoac\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2838-7211","authenticated-orcid":false,"given":"V\u00edctor","family":"Meza-Carmen","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Morelia, Michoac\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Silva-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n sobre Enfermedades Infecciosas, Instituto Nacional de Salud P\u00fablica, Cuernavaca, Morelos, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha I.","family":"Ram\u00edrez-D\u00edaz","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico-Biol\u00f3gicas, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Morelia, Michoac\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21040268"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi5000564"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg208"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.12830"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2014.04.007"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2008.00063.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2004.11.012"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1347"},{"key":"e_1_3_3_10_2","first-page":"159","article-title":"Resistance to metals by Pseudomonas aeruginosa clinical isolates","volume":"48","author":"Cervantes-Vega C","year":"1986","unstructured":"Cervantes-Vega C, Ch\u00e1vez J, C\u00f3rdova NA, De la Mora P, Velasco JA. 1986. 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