{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T22:24:33Z","timestamp":1780093473653,"version":"3.54.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2014R1A1A1007464.1"],"award-info":[{"award-number":["2014R1A1A1007464.1"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016R1D1A1A02937214"],"award-info":[{"award-number":["2016R1D1A1A02937214"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2014K1A4A7A01074643"],"award-info":[{"award-number":["2014K1A4A7A01074643"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            New and improved treatments for tuberculosis (TB) are urgently needed. Recently, it has been demonstrated that verapamil, an efflux inhibitor, can reduce bacterial drug tolerance caused by efflux pump activity when administered in combination with available antituberculosis agents. The aim of this study was to evaluate the effectiveness of verapamil in combination with the antituberculosis drug candidate Q203, which has recently been developed and is currently under clinical trials as a potential antituberculosis agent. We evaluated changes in Q203 activity in the presence and absence of verapamil\n            <jats:italic>in vitro<\/jats:italic>\n            using the resazurin microplate assay and\n            <jats:italic>ex vivo<\/jats:italic>\n            using a microscopy-based phenotypic assay for the quantification of intracellular replicating mycobacteria. Verapamil increased the potency of Q203 against\n            <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n            both\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>ex vivo<\/jats:italic>\n            , indicating that efflux pumps are associated with the activity of Q203. Other efflux pump inhibitors also displayed an increase in Q203 potency, strengthening this hypothesis. Therefore, the combination of verapamil and Q203 may be a promising combinatorial strategy for anti-TB treatment to accelerate the elimination of\n            <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/aac.02637-16","type":"journal-article","created":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T00:55:21Z","timestamp":1492476921000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Efflux Attenuates the Antibacterial Activity of Q203 in Mycobacterium tuberculosis"],"prefix":"10.1128","volume":"61","author":[{"given":"Jichan","family":"Jang","sequence":"first","affiliation":[{"name":"Tuberculosis Research Laboratory, Institut Pasteur Korea, Seongnam, Gyeonggi, South Korea"},{"name":"Division of Life Science, Gyeongsang National University, Jinju, South Korea"},{"name":"Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea"},{"name":"Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryangyeo","family":"Kim","sequence":"additional","affiliation":[{"name":"Tuberculosis Research Laboratory, Institut Pasteur Korea, Seongnam, Gyeonggi, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjeong","family":"Woo","sequence":"additional","affiliation":[{"name":"Tuberculosis Research Laboratory, Institut Pasteur Korea, Seongnam, Gyeonggi, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinsun","family":"Jeong","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da Eun","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guehye","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Delorme","sequence":"additional","affiliation":[{"name":"Tuberculosis Research Laboratory, Institut Pasteur Korea, Seongnam, Gyeonggi, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"World Health Organization. 2016. Global tuberculosis report 2016. World Health Organization Geneva Switzerland. http:\/\/www.who.int\/tb\/publications\/global_report\/en\/."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903856"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2009.02.005"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.06.035345"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2011.00831.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2011.07.001"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/651377"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034538"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.022"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201304-0650OC"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01462-13"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04019-14"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3262"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000645"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu095"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio884"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01482-12"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0102135"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00037-14"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02087-16"},{"key":"e_1_3_3_22_2","first-page":"684","article-title":"Unexpected high prevalence of resistance-associated Rv0678 variants in MDR-TB patients without documented prior use of clofazimine or bedaquiline","volume":"72","author":"Villellas C","year":"2017","unstructured":"Villellas C CoeckN MeehanCJ LounisN de JongB RigoutsL AndriesK. 2017. Unexpected high prevalence of resistance-associated Rv0678 variants in MDR-TB patients without documented prior use of clofazimine or bedaquiline. J Antimicrob Chemother72:684\u2013690.","journal-title":"J Antimicrob Chemother"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.025734-0"}],"container-title":["Antimicrobial Agents and Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02637-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AAC.02637-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T21:07:05Z","timestamp":1645477625000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AAC.02637-16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":22,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1128\/AAC.02637-16"],"URL":"https:\/\/doi.org\/10.1128\/aac.02637-16","relation":{},"ISSN":["0066-4804","1098-6596"],"issn-type":[{"value":"0066-4804","type":"print"},{"value":"1098-6596","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7]]},"assertion":[{"value":"2016-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-03-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-06-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02637-16"}}