{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T02:00:50Z","timestamp":1775959250874,"version":"3.50.1"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2014,11,1]],"date-time":"2014-11-01T00:00:00Z","timestamp":1414800000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Antimicrob Agents Chemother"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Toxoplasma gondii<\/jats:named-content>\n            is a major food pathogen and neglected parasitic infection that causes eye disease, birth defects, and fetal abortion and plays a role as an opportunistic infection in AIDS. In this study, we investigated pantothenic acid (vitamin B\n            <jats:sub>5<\/jats:sub>\n            ) biosynthesis in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">T. gondii<\/jats:named-content>\n            . Genes encoding the full repertoire of enzymes for pantothenate synthesis and subsequent metabolism to coenzyme A were identified and are expressed in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">T. gondii<\/jats:named-content>\n            . A panel of inhibitors developed to target\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Mycobacterium tuberculosis<\/jats:named-content>\n            pantothenate synthetase were tested and found to exhibit a range of values for inhibition of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">T. gondii<\/jats:named-content>\n            growth. Two inhibitors exhibited lower effective concentrations than the currently used toxoplasmosis drug pyrimethamine. The inhibition was specific for the pantothenate pathway, as the effect of the pantothenate synthetase inhibitors was abrogated by supplementation with pantothenate. Hence,\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">T. gondii<\/jats:named-content>\n            encodes and expresses the enzymes for pantothenate synthesis, and this pathway is essential for parasite growth. These promising findings increase our understanding of growth and metabolism in this important parasite and highlight pantothenate synthetase as a new drug target.\n          <\/jats:p>","DOI":"10.1128\/aac.02640-14","type":"journal-article","created":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T03:15:21Z","timestamp":1405998921000},"page":"6345-6353","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Pantothenic Acid Biosynthesis in the Parasite Toxoplasma gondii: a Target for Chemotherapy"],"prefix":"10.1128","volume":"58","author":[{"given":"Sarmad N.","family":"Mageed","sequence":"first","affiliation":[{"name":"Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fraser","family":"Cunningham","sequence":"additional","affiliation":[{"name":"Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvin Wei","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hernani Leonardo","family":"Silvestre","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shijun","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom L.","family":"Blundell","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"Abell","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenn A.","family":"McConkey","sequence":"additional","affiliation":[{"name":"Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Ranking the risks: the 10 pathogen-food combinations with the greatest burden on public health","author":"Batz MB","year":"2011","unstructured":"BatzMBHoffmannSMorrisJGJr. 2011. 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